tetano
Editor, Senior Moderator
J Med Virol
. 2023 Dec;95(12):e29268.
doi: 10.1002/jmv.29268. Omicron induced distinct immune respiratory transcriptomics signatures compared to pre-existing variants in critically ill COVID-19 patients
Pierre Bay[SUP] 1 2 3 [/SUP], Christophe Rodriguez[SUP] 3 4 5 [/SUP], Stefano Caruso[SUP] 3 6 [/SUP], Vanessa Demontant[SUP] 5 [/SUP], Laure Boizeau[SUP] 5 [/SUP], Alexandre Soulier[SUP] 3 4 [/SUP], Paul L Woerther[SUP] 4 7 [/SUP], Armand Mekontso-Dessap[SUP] 1 2 [/SUP], Jean-Michel Pawlotsky[SUP] 3 4 5 [/SUP], Nicolas de Prost[SUP] 1 2 [/SUP], Slim Fourati[SUP] 3 4 [/SUP]
Affiliations
Severe coronavirus disease 2019 (COVID-19) is related to dysregulated immune responses. We aimed to explore the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants on the immune response by nasopharyngeal transcriptomic in critically-ill patients. This prospective monocentric study included COVID-19 patients requiring intensive care unit (ICU) admission between March 2020 and 2022. Patients were classified according to VOC (ancestral, Alpha, Delta, and Omicron). Eighty-eight patients with severe COVID-19 were included after matching (on prespecified clinical criteria). Profiling of gene expression markers of innate and adaptive immune responses were investigated by respiratory transcriptomics at ICU admission. Eighty-eight patients were included in the study after matching (ancestral [n = 24], Alpha [n = 24], Delta [n = 22], and Omicron [n = 18] variants). Respiratory transcriptomic analysis revealed distinct innate and adaptive immune profiling between variants. In comparison with the ancestral variant, there was a reduced expression of neutrophil degranulation, T cell activation, cytokines signalling pathways in patients infected with Alpha and Delta variants. In contrast, there was a higher expression of neutrophil degranulation, T and B cells activation, and inflammatory interleukins pathways in patients infected with Omicron. To conclude, Omicron induced distinct immune respiratory transcriptomics signatures compared to pre-existing variants in patients with severe COVID-19, pointing to an evolving pathophysiology of severe COVID-19 in the Omicron era.
Keywords: SARS-CoV-2; acute respiratory distress syndrome; immune response; intensive care unit; omicron; transcriptomics; variants.
. 2023 Dec;95(12):e29268.
doi: 10.1002/jmv.29268. Omicron induced distinct immune respiratory transcriptomics signatures compared to pre-existing variants in critically ill COVID-19 patients
Pierre Bay[SUP] 1 2 3 [/SUP], Christophe Rodriguez[SUP] 3 4 5 [/SUP], Stefano Caruso[SUP] 3 6 [/SUP], Vanessa Demontant[SUP] 5 [/SUP], Laure Boizeau[SUP] 5 [/SUP], Alexandre Soulier[SUP] 3 4 [/SUP], Paul L Woerther[SUP] 4 7 [/SUP], Armand Mekontso-Dessap[SUP] 1 2 [/SUP], Jean-Michel Pawlotsky[SUP] 3 4 5 [/SUP], Nicolas de Prost[SUP] 1 2 [/SUP], Slim Fourati[SUP] 3 4 [/SUP]
Affiliations
- PMID: 38050838
- DOI: 10.1002/jmv.29268
Severe coronavirus disease 2019 (COVID-19) is related to dysregulated immune responses. We aimed to explore the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants on the immune response by nasopharyngeal transcriptomic in critically-ill patients. This prospective monocentric study included COVID-19 patients requiring intensive care unit (ICU) admission between March 2020 and 2022. Patients were classified according to VOC (ancestral, Alpha, Delta, and Omicron). Eighty-eight patients with severe COVID-19 were included after matching (on prespecified clinical criteria). Profiling of gene expression markers of innate and adaptive immune responses were investigated by respiratory transcriptomics at ICU admission. Eighty-eight patients were included in the study after matching (ancestral [n = 24], Alpha [n = 24], Delta [n = 22], and Omicron [n = 18] variants). Respiratory transcriptomic analysis revealed distinct innate and adaptive immune profiling between variants. In comparison with the ancestral variant, there was a reduced expression of neutrophil degranulation, T cell activation, cytokines signalling pathways in patients infected with Alpha and Delta variants. In contrast, there was a higher expression of neutrophil degranulation, T and B cells activation, and inflammatory interleukins pathways in patients infected with Omicron. To conclude, Omicron induced distinct immune respiratory transcriptomics signatures compared to pre-existing variants in patients with severe COVID-19, pointing to an evolving pathophysiology of severe COVID-19 in the Omicron era.
Keywords: SARS-CoV-2; acute respiratory distress syndrome; immune response; intensive care unit; omicron; transcriptomics; variants.