tetano
Editor, Senior Moderator
J Vet Sci
. 2021 Aug 9.
doi: 10.4142/jvs.2021.22.e70. Online ahead of print.
Molecular detection of bat coronaviruses in three bat species in Indonesia
Ni Luh Putu Indi Dharmayanti[SUP] #[/SUP][SUP] 1 [/SUP], Diana Nurjanah[SUP] #[/SUP][SUP] 2 [/SUP], Harimurti Nuradji[SUP] 2 [/SUP], Ibnu Maryanto[SUP] 3 [/SUP], Indra Exploitasia[SUP] 4 [/SUP], Risa Indriani[SUP] 2 [/SUP]
Affiliations
Abstract
Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.
Keywords: Coronavirus; Indonesia; bats; zoonoses.
. 2021 Aug 9.
doi: 10.4142/jvs.2021.22.e70. Online ahead of print.
Molecular detection of bat coronaviruses in three bat species in Indonesia
Ni Luh Putu Indi Dharmayanti[SUP] #[/SUP][SUP] 1 [/SUP], Diana Nurjanah[SUP] #[/SUP][SUP] 2 [/SUP], Harimurti Nuradji[SUP] 2 [/SUP], Ibnu Maryanto[SUP] 3 [/SUP], Indra Exploitasia[SUP] 4 [/SUP], Risa Indriani[SUP] 2 [/SUP]
Affiliations
- PMID: 34697920
- DOI: 10.4142/jvs.2021.22.e70
Abstract
Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.
Keywords: Coronavirus; Indonesia; bats; zoonoses.