Giuseppe
Emeritus
J Virol. 2009 Jul 8. [Epub ahead of print]
A "One-Plasmid" System to Generate Influenza Virus in Cultured Chicken Cells for Potential Use in Flu Vaccine.
Zhang X, Kong W, Ashraf S, Curtiss R 3rd. Center for Infectious Diseases and Vaccinology, The Biodesign Institute, and School of Life Science, Arizona State University, Tempe, Arizona 85287.
Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza polymerase subunits and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters we constructed a 23.6 kb 8-unit plasmid that contains all the required constituents to generate influenza virus in chicken cells. Our "one-plasmid" system generated higher titers of influenza virus in chicken cells than the "eight-plasmid" system, enabling a simpler approach for generating vaccine seeds. Our study identified plasmid size as a potential limiting factor affecting transfection efficiency and hence the influenza viral yield from chicken cell.
PMID: 19587040 [PubMed - as supplied by publishe
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A "One-Plasmid" System to Generate Influenza Virus in Cultured Chicken Cells for Potential Use in Flu Vaccine.
Zhang X, Kong W, Ashraf S, Curtiss R 3rd. Center for Infectious Diseases and Vaccinology, The Biodesign Institute, and School of Life Science, Arizona State University, Tempe, Arizona 85287.
Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza polymerase subunits and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters we constructed a 23.6 kb 8-unit plasmid that contains all the required constituents to generate influenza virus in chicken cells. Our "one-plasmid" system generated higher titers of influenza virus in chicken cells than the "eight-plasmid" system, enabling a simpler approach for generating vaccine seeds. Our study identified plasmid size as a potential limiting factor affecting transfection efficiency and hence the influenza viral yield from chicken cell.
PMID: 19587040 [PubMed - as supplied by publishe
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