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J Virol . A Single Vaccine Protects against SARS-CoV-2 and Influenza Virus in Mice

tetano

Editor, Senior Moderator
J Virol


. 2021 Dec 15;JVI0157821.
doi: 10.1128/JVI.01578-21. Online ahead of print.
A Single Vaccine Protects against SARS-CoV-2 and Influenza Virus in Mice


Kangli Cao[SUP] 1 2 [/SUP], Xiang Wang[SUP] 2 [/SUP], Haoran Peng[SUP] 3 [/SUP], Longfei Ding[SUP] 2 [/SUP], Xiangwei Wang[SUP] 2 [/SUP], Yangyang Hu[SUP] 2 [/SUP], Lanlan Dong[SUP] 1 [/SUP], Tianhan Yang[SUP] 2 [/SUP], Xiujing Hong[SUP] 1 [/SUP], Man Xing[SUP] 4 [/SUP], Duoduo Li[SUP] 2 [/SUP], Cuisong Zhu[SUP] 2 [/SUP], Xiangchuan He[SUP] 1 [/SUP], Chen Zhao[SUP] 2 [/SUP], Ping Zhao[SUP] 3 [/SUP], Dongming Zhou[SUP] 2 4 [/SUP], Xiaoyan Zhang[SUP] 1 2 [/SUP], Jianqing Xu[SUP] 1 2 [/SUP]



Affiliations

Abstract

The ongoing SARS-CoV-2 pandemic posed a severe global threat on public health, as do so by influenza viruses (influenza) and other coronaviruses. Here we present chimpanzee adenovirus 68 (AdC68)-based vaccines designed to universally target coronaviruses and influenza. Our design is centered on an immunogen generated by fusing the SARS-CoV-2 receptor-binding domain (RBD) to the conserved stalk of H7N9 hemagglutinin (HA). Remarkably, the constructed vaccine effectively induced both SARS-CoV-2-targeting antibodies and anti-influenza antibodies in mice, consequently affording protection from lethal SARS-CoV-2 and H7N9 challenges and effective H3N2 control. We propose our AdC68 vectored coronavirus-influenza vaccine as a universal approach toward curbing respiratory virus-causing pandemics. IMPORTANCE The COVID-19 pandemic exemplifies the severe public health threat of respiratory virus infection, as do so by influenza A viruses. The currently envisioned strategy for prevention of respiratory virus-causing diseases requires comprehensive administration of vaccines tailored for individual virus. Here we present an alternative strategy by designing chimpanzee adenovirus 68-based vaccines targeting both SARS-CoV-2 receptor-binding-domain and conserved stalk of influenza hemagglutinin. When tested in mice, this strategy attained potent neutralizing antibodies against wild-type SARS-CoV-2 as well as its emerging variants, enabling an effective protection against lethal SARS-CoV-2 challenge. Notably, it also entitled a complete protection from lethal H7N9 challenge and efficient control of H3N2-induced morbidity. Our study opens a new avenue to universally curb respiratory virus infection by vaccination.
 
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