tetano
Editor, Senior Moderator
J Virol
. 2024 Oct 10:e0138524.
doi: 10.1128/jvi.01385-24. Online ahead of print. Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions
Joseph D Trimarco[SUP] 1 [/SUP], M Ariel Spurrier[SUP] 1 [/SUP], Samantha Skavicus[SUP] 1 [/SUP], Zhaochen Luo[SUP] 1 [/SUP], Moumita Dutta[SUP] 2 [/SUP], Katarzyna Janowska[SUP] 2 [/SUP], Priyamvada Acharya[SUP] 2 3 4 [/SUP], Brook E Heaton[SUP] 1 [/SUP], Nicholas S Heaton[SUP] 1 2 5 [/SUP]
Affiliations
In early 2024, a clade 2.3.4.4b high pathogenic H5N1 avian influenza virus was detected in dairy cows and humans in the United States. Since then, it has spread to herds in at least 13 states and caused symptomatic disease in at least fifteen people. To facilitate rapid testing of existing and novel countermeasures, here, we report the development of an H5N1 viral reverse genetic system, its use to produce fluorescent and bioluminescent variant strains, and their utility in high-throughput evaluation of antiviral interventions.
Keywords: NanoLuc; drug testing; influenza viruses; sfGFP.
. 2024 Oct 10:e0138524.
doi: 10.1128/jvi.01385-24. Online ahead of print. Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions
Joseph D Trimarco[SUP] 1 [/SUP], M Ariel Spurrier[SUP] 1 [/SUP], Samantha Skavicus[SUP] 1 [/SUP], Zhaochen Luo[SUP] 1 [/SUP], Moumita Dutta[SUP] 2 [/SUP], Katarzyna Janowska[SUP] 2 [/SUP], Priyamvada Acharya[SUP] 2 3 4 [/SUP], Brook E Heaton[SUP] 1 [/SUP], Nicholas S Heaton[SUP] 1 2 5 [/SUP]
Affiliations
- PMID: 39387583
- DOI: 10.1128/jvi.01385-24
In early 2024, a clade 2.3.4.4b high pathogenic H5N1 avian influenza virus was detected in dairy cows and humans in the United States. Since then, it has spread to herds in at least 13 states and caused symptomatic disease in at least fifteen people. To facilitate rapid testing of existing and novel countermeasures, here, we report the development of an H5N1 viral reverse genetic system, its use to produce fluorescent and bioluminescent variant strains, and their utility in high-throughput evaluation of antiviral interventions.
Keywords: NanoLuc; drug testing; influenza viruses; sfGFP.