tetano
Editor, Senior Moderator
J Virol
. 2020 Dec 21;JVI.02002-20.
doi: 10.1128/JVI.02002-20. Online ahead of print.
Identification of SARS-CoV-2 Nucleocapsid and Spike T-cell Epitopes for Assessing T-cell Immunity
Eunok Lee[SUP] 1 2 [/SUP], Kerrie Sandgren[SUP] 3 2 [/SUP], Gabriel Duette[SUP] 3 2 [/SUP], Vicki V Stylianou[SUP] 3 2 [/SUP], Rajiv Khanna[SUP] 4 5 [/SUP], John-Sebastian Eden[SUP] 3 2 6 7 [/SUP], Emily Blyth[SUP] 2 8 9 [/SUP], David Gottlieb[SUP] 2 8 9 [/SUP], Anthony L Cunningham[SUP] 3 2 [/SUP], Sarah Palmer[SUP] 3 2 [/SUP]
Affiliations
Abstract
Developing optimal T-cell response assays to severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is critical for measuring the duration of immunity to this disease and assessing the efficacy of vaccine candidates. These assays need to target conserved regions of SARS-CoV-2 global variants and avoid cross-reactivity to seasonal human coronaviruses. To contribute to this effort, we employed an in-silico immunoinformatics analysis pipeline to identify immunogenic peptides resulting from conserved and highly networked regions with topological importance from the SARS-CoV-2 nucleocapsid and spike proteins. A total of 57 highly networked T-cell epitopes that are conserved across geographic viral variants were identified from these viral proteins, with a binding potential to diverse HLA alleles and 80-100% global population coverage. Importantly, 18 of these T-cell epitope derived peptides had limited homology to seasonal human coronaviruses making them promising candidates for SARS-CoV-2 specific T-cell immunity assays. Moreover, two of the NC-derived peptides elicited effector/polyfunctional responses of CD8+ T-cells derived from SARS-CoV-2 convalescent patients.ImportanceThe development of specific and validated immunologic tools is critical for understanding the level and duration of the cellular response induced by SARS-CoV-2 infection and/or vaccines against this novel coronavirus disease. To contribute to this effort, we employed an immunoinformatics analysis pipeline to define 57 SARS-CoV-2 immunogenic peptides within topologically important regions of the nucleocapsid (NC) and spike (S) proteins that will be effective for detecting cellular immune responses in 80-100% of the global population. Our immunoinformatics analysis revealed that 18 of these peptides had limited homology to circulating seasonal human coronaviruses, and therefore are promising candidates for distinguishing SARS-CoV-2 specific immune responses from pre-existing coronavirus immunity. Importantly, CD8+ T-cells derived from SARS-CoV-2 survivors exhibited polyfunctional effector responses to two novel NC-derived peptides identified as HLA-binders. These studies provide a proof of concept that our immunoinformatics analysis pipeline identifies novel immunogens which can elicit polyfunctional SARS-CoV-2 specific T-cell responses.
. 2020 Dec 21;JVI.02002-20.
doi: 10.1128/JVI.02002-20. Online ahead of print.
Identification of SARS-CoV-2 Nucleocapsid and Spike T-cell Epitopes for Assessing T-cell Immunity
Eunok Lee[SUP] 1 2 [/SUP], Kerrie Sandgren[SUP] 3 2 [/SUP], Gabriel Duette[SUP] 3 2 [/SUP], Vicki V Stylianou[SUP] 3 2 [/SUP], Rajiv Khanna[SUP] 4 5 [/SUP], John-Sebastian Eden[SUP] 3 2 6 7 [/SUP], Emily Blyth[SUP] 2 8 9 [/SUP], David Gottlieb[SUP] 2 8 9 [/SUP], Anthony L Cunningham[SUP] 3 2 [/SUP], Sarah Palmer[SUP] 3 2 [/SUP]
Affiliations
- PMID: 33443088
- DOI: 10.1128/JVI.02002-20
Abstract
Developing optimal T-cell response assays to severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is critical for measuring the duration of immunity to this disease and assessing the efficacy of vaccine candidates. These assays need to target conserved regions of SARS-CoV-2 global variants and avoid cross-reactivity to seasonal human coronaviruses. To contribute to this effort, we employed an in-silico immunoinformatics analysis pipeline to identify immunogenic peptides resulting from conserved and highly networked regions with topological importance from the SARS-CoV-2 nucleocapsid and spike proteins. A total of 57 highly networked T-cell epitopes that are conserved across geographic viral variants were identified from these viral proteins, with a binding potential to diverse HLA alleles and 80-100% global population coverage. Importantly, 18 of these T-cell epitope derived peptides had limited homology to seasonal human coronaviruses making them promising candidates for SARS-CoV-2 specific T-cell immunity assays. Moreover, two of the NC-derived peptides elicited effector/polyfunctional responses of CD8+ T-cells derived from SARS-CoV-2 convalescent patients.ImportanceThe development of specific and validated immunologic tools is critical for understanding the level and duration of the cellular response induced by SARS-CoV-2 infection and/or vaccines against this novel coronavirus disease. To contribute to this effort, we employed an immunoinformatics analysis pipeline to define 57 SARS-CoV-2 immunogenic peptides within topologically important regions of the nucleocapsid (NC) and spike (S) proteins that will be effective for detecting cellular immune responses in 80-100% of the global population. Our immunoinformatics analysis revealed that 18 of these peptides had limited homology to circulating seasonal human coronaviruses, and therefore are promising candidates for distinguishing SARS-CoV-2 specific immune responses from pre-existing coronavirus immunity. Importantly, CD8+ T-cells derived from SARS-CoV-2 survivors exhibited polyfunctional effector responses to two novel NC-derived peptides identified as HLA-binders. These studies provide a proof of concept that our immunoinformatics analysis pipeline identifies novel immunogens which can elicit polyfunctional SARS-CoV-2 specific T-cell responses.