tetano
Editor, Senior Moderator
J Virol Methods
. 2024 Mar 5:114909.
doi: 10.1016/j.jviromet.2024.114909. Online ahead of print. Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples
Takashi Katsuno[SUP] 1 [/SUP], Moto Kimura[SUP] 2 [/SUP], Junko Terada-Hirashima[SUP] 3 [/SUP], Yukumasa Kazuyama[SUP] 4 [/SUP], Masato Ikeda[SUP] 4 [/SUP], Ataru Moriya[SUP] 5 [/SUP], Masami Kurokawa[SUP] 5 [/SUP], Ayano Motohashi[SUP] 5 [/SUP], Erina Isaka[SUP] 5 [/SUP], Momoko Morishita[SUP] 3 [/SUP], Kazuki Kawajiri[SUP] 3 [/SUP], Kazuo Hakkaku[SUP] 3 [/SUP], Susumu Saito[SUP] 3 [/SUP], Yuriko Terayama[SUP] 3 [/SUP], Yuriko Sugiura[SUP] 3 [/SUP], Yoh Yamaguchi[SUP] 3 [/SUP], Hiroshi Takumida[SUP] 3 [/SUP], Hiromu Watanabe[SUP] 3 [/SUP], Chie Morita[SUP] 3 [/SUP], Akinari Tsukada[SUP] 3 [/SUP], Yusaku Kusaba[SUP] 3 [/SUP], Yoshie Tsujimoto[SUP] 3 [/SUP], Akane Ishida[SUP] 3 [/SUP], Keita Sakamoto[SUP] 3 [/SUP], Masao Hashimoto[SUP] 3 [/SUP], Manabu Suzuki[SUP] 3 [/SUP], Jin Takasaki[SUP] 3 [/SUP], Shinyu Izumi[SUP] 3 [/SUP], Masayuki Hojo[SUP] 3 [/SUP], Haruhito Sugiyama[SUP] 3 [/SUP], Wataru Sugiura[SUP] 2 [/SUP]
Affiliations
This study aimed to evaluate diagnostic accuracy of SARS-CoV-2 RNA detection in saliva samples treated with a guanidine-based or guanidine-free inactivator, using nasopharyngeal swab samples (NPS) as referents. Based on the NPS reverse transcription-polymerase chain reaction (RT-PCR) results, participants were classified as with or without COVID-19. Fifty sets of samples comprising NPS, self-collected raw saliva, and saliva with a guanidine-based, and guanidine-free inactivator were collected from each group. In patients with COVID-19, the sensitivity of direct RT-PCR using raw saliva and saliva treated with a guanidine-based and guanidine-free inactivator was 100.0%, 65.9%, and 82.9%, respectively, with corresponding concordance rates of 94.3% (κ=88.5), 82.8% (κ=64.8), and 92.0% (κ=83.7). Among patients with a PCR Ct value of <30 in the NPS sample, the positive predictive value for the three samples was 100.0%, 80.0%, and 96.0%, respectively. The sensitivity of SARS-CoV-2 RNA detection was lower in inactivated saliva than in raw saliva and lower in samples treated with a guanidine-based than with a guanidine-free inactivator. However, in individuals contributing to infection spread, inactivated saliva showed adequate accuracy regardless of the inactivator used. Inactivators can be added to saliva samples collected for RT-PCR to reduce viral transmission risk while maintaining adequate diagnostic accuracy.
Keywords: COVID-19; SARS-CoV-2; guanidine; inactivator; polymerase chain reaction; saliva.
. 2024 Mar 5:114909.
doi: 10.1016/j.jviromet.2024.114909. Online ahead of print. Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples
Takashi Katsuno[SUP] 1 [/SUP], Moto Kimura[SUP] 2 [/SUP], Junko Terada-Hirashima[SUP] 3 [/SUP], Yukumasa Kazuyama[SUP] 4 [/SUP], Masato Ikeda[SUP] 4 [/SUP], Ataru Moriya[SUP] 5 [/SUP], Masami Kurokawa[SUP] 5 [/SUP], Ayano Motohashi[SUP] 5 [/SUP], Erina Isaka[SUP] 5 [/SUP], Momoko Morishita[SUP] 3 [/SUP], Kazuki Kawajiri[SUP] 3 [/SUP], Kazuo Hakkaku[SUP] 3 [/SUP], Susumu Saito[SUP] 3 [/SUP], Yuriko Terayama[SUP] 3 [/SUP], Yuriko Sugiura[SUP] 3 [/SUP], Yoh Yamaguchi[SUP] 3 [/SUP], Hiroshi Takumida[SUP] 3 [/SUP], Hiromu Watanabe[SUP] 3 [/SUP], Chie Morita[SUP] 3 [/SUP], Akinari Tsukada[SUP] 3 [/SUP], Yusaku Kusaba[SUP] 3 [/SUP], Yoshie Tsujimoto[SUP] 3 [/SUP], Akane Ishida[SUP] 3 [/SUP], Keita Sakamoto[SUP] 3 [/SUP], Masao Hashimoto[SUP] 3 [/SUP], Manabu Suzuki[SUP] 3 [/SUP], Jin Takasaki[SUP] 3 [/SUP], Shinyu Izumi[SUP] 3 [/SUP], Masayuki Hojo[SUP] 3 [/SUP], Haruhito Sugiyama[SUP] 3 [/SUP], Wataru Sugiura[SUP] 2 [/SUP]
Affiliations
- PMID: 38452822
- DOI: 10.1016/j.jviromet.2024.114909
This study aimed to evaluate diagnostic accuracy of SARS-CoV-2 RNA detection in saliva samples treated with a guanidine-based or guanidine-free inactivator, using nasopharyngeal swab samples (NPS) as referents. Based on the NPS reverse transcription-polymerase chain reaction (RT-PCR) results, participants were classified as with or without COVID-19. Fifty sets of samples comprising NPS, self-collected raw saliva, and saliva with a guanidine-based, and guanidine-free inactivator were collected from each group. In patients with COVID-19, the sensitivity of direct RT-PCR using raw saliva and saliva treated with a guanidine-based and guanidine-free inactivator was 100.0%, 65.9%, and 82.9%, respectively, with corresponding concordance rates of 94.3% (κ=88.5), 82.8% (κ=64.8), and 92.0% (κ=83.7). Among patients with a PCR Ct value of <30 in the NPS sample, the positive predictive value for the three samples was 100.0%, 80.0%, and 96.0%, respectively. The sensitivity of SARS-CoV-2 RNA detection was lower in inactivated saliva than in raw saliva and lower in samples treated with a guanidine-based than with a guanidine-free inactivator. However, in individuals contributing to infection spread, inactivated saliva showed adequate accuracy regardless of the inactivator used. Inactivators can be added to saliva samples collected for RT-PCR to reduce viral transmission risk while maintaining adequate diagnostic accuracy.
Keywords: COVID-19; SARS-CoV-2; guanidine; inactivator; polymerase chain reaction; saliva.