tetano
Editor, Senior Moderator
J Vis Exp
. 2020 Jun 27;(160).
doi: 10.3791/60523.
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Meng Wang[SUP] #[/SUP][SUP] 1 [/SUP], Yuanyuan Huang[SUP] #[/SUP][SUP] 1 [/SUP], Wangpeng Gu[SUP] 2 [/SUP], Haikun Wang[SUP] 3 [/SUP]
Affiliations
Abstract
T Follicular Helper (Tfh) cells are an independent CD4[SUP]+ [/SUP]T cell subset specialized in providing help for germinal center (GC) development and generation of high-affinity antibodies. In influenza virus infection, robust Tfh and GC B cell responses are induced to facilitate effective virus eradication, which confers a qualified mouse model for Tfh-associated study. In this paper, we described protocols in detection of basic Tfh-associated immune response during influenza virus infection in mice. These protocols include: intranasal inoculation of influenza virus; flow cytometry staining and analysis of polyclonal and antigen-specific Tfh cells, GC B cells and plasma cells; immunofluorescence detection of GCs; enzyme-linked immunosorbent assay (ELISA) of influenza virus-specific antibody in serum. These assays basically quantify the differentiation and function of Tfh cells in influenza virus infection, thus providing help for studies in elucidating differentiation mechanism and manipulation strategy.
. 2020 Jun 27;(160).
doi: 10.3791/60523.
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Meng Wang[SUP] #[/SUP][SUP] 1 [/SUP], Yuanyuan Huang[SUP] #[/SUP][SUP] 1 [/SUP], Wangpeng Gu[SUP] 2 [/SUP], Haikun Wang[SUP] 3 [/SUP]
Affiliations
- PMID: 32658200
- DOI: 10.3791/60523
Abstract
T Follicular Helper (Tfh) cells are an independent CD4[SUP]+ [/SUP]T cell subset specialized in providing help for germinal center (GC) development and generation of high-affinity antibodies. In influenza virus infection, robust Tfh and GC B cell responses are induced to facilitate effective virus eradication, which confers a qualified mouse model for Tfh-associated study. In this paper, we described protocols in detection of basic Tfh-associated immune response during influenza virus infection in mice. These protocols include: intranasal inoculation of influenza virus; flow cytometry staining and analysis of polyclonal and antigen-specific Tfh cells, GC B cells and plasma cells; immunofluorescence detection of GCs; enzyme-linked immunosorbent assay (ELISA) of influenza virus-specific antibody in serum. These assays basically quantify the differentiation and function of Tfh cells in influenza virus infection, thus providing help for studies in elucidating differentiation mechanism and manipulation strategy.