tetano
Editor, Senior Moderator
J Water Health
. 2024 Jun;22(6):978-992.
doi: 10.2166/wh.2024.303. Epub 2024 Apr 12. Monitoring SARS-CoV-2 RNA in wastewater from a shared septic system and sub-sewershed sites to expand COVID-19 disease surveillance
A B Tanvir Pasha[SUP] 1 [/SUP], Nadine Kotlarz[SUP] 2 [/SUP], David Holcomb[SUP] 3 [/SUP], Stacie Reckling[SUP] 4 [/SUP], Judith Kays[SUP] 1 [/SUP], Erika Bailey[SUP] 5 [/SUP], Virginia Guidry[SUP] 4 [/SUP], Ariel Christensen[SUP] 4 [/SUP], Steven Berkowitz[SUP] 4 [/SUP], Lawrence S Engel[SUP] 3 [/SUP], Francis de Los Reyes[SUP] 1 [/SUP], Angela Harris[SUP] 6 [/SUP]
Affiliations
Wastewater-based epidemiology has expanded as a tool for collecting COVID-19 surveillance data, but there is limited information on the feasibility of this form of surveillance within decentralized wastewater systems (e.g., septic systems). This study assessed SARS-CoV-2 RNA concentrations in wastewater samples from a septic system servicing a mobile home park (66 households) and from two pumping stations serving a similarly sized (71 households) and a larger (1,000 households) neighborhood within a nearby sewershed over 35 weeks in 2020. Also, raw wastewater from a hospital in the same sewershed was sampled. The mobile home park samples had the highest detection frequency (39/39 days) and mean concentration of SARS-CoV-2 RNA (2.7 × 10[SUP]7[/SUP] gene copies/person/day for the N1) among the four sampling sites. N1 gene and N2 gene copies were highly correlated across mobile home park samples (Pearson's r = 0.93, p < 0.0001). In the larger neighborhood, new COVID-19 cases were reported every week during the sampling period; however, we detected SARS-CoV-2 RNA in 12% of the corresponding wastewater samples. The results of this study suggest that sampling from decentralized wastewater infrastructure can be used for continuous monitoring of SARS-CoV-2 infections.
Keywords: SARS-CoV-2; septic tanks; wastewater-based epidemiology.
. 2024 Jun;22(6):978-992.
doi: 10.2166/wh.2024.303. Epub 2024 Apr 12. Monitoring SARS-CoV-2 RNA in wastewater from a shared septic system and sub-sewershed sites to expand COVID-19 disease surveillance
A B Tanvir Pasha[SUP] 1 [/SUP], Nadine Kotlarz[SUP] 2 [/SUP], David Holcomb[SUP] 3 [/SUP], Stacie Reckling[SUP] 4 [/SUP], Judith Kays[SUP] 1 [/SUP], Erika Bailey[SUP] 5 [/SUP], Virginia Guidry[SUP] 4 [/SUP], Ariel Christensen[SUP] 4 [/SUP], Steven Berkowitz[SUP] 4 [/SUP], Lawrence S Engel[SUP] 3 [/SUP], Francis de Los Reyes[SUP] 1 [/SUP], Angela Harris[SUP] 6 [/SUP]
Affiliations
- PMID: 38935450
- DOI: 10.2166/wh.2024.303
Wastewater-based epidemiology has expanded as a tool for collecting COVID-19 surveillance data, but there is limited information on the feasibility of this form of surveillance within decentralized wastewater systems (e.g., septic systems). This study assessed SARS-CoV-2 RNA concentrations in wastewater samples from a septic system servicing a mobile home park (66 households) and from two pumping stations serving a similarly sized (71 households) and a larger (1,000 households) neighborhood within a nearby sewershed over 35 weeks in 2020. Also, raw wastewater from a hospital in the same sewershed was sampled. The mobile home park samples had the highest detection frequency (39/39 days) and mean concentration of SARS-CoV-2 RNA (2.7 × 10[SUP]7[/SUP] gene copies/person/day for the N1) among the four sampling sites. N1 gene and N2 gene copies were highly correlated across mobile home park samples (Pearson's r = 0.93, p < 0.0001). In the larger neighborhood, new COVID-19 cases were reported every week during the sampling period; however, we detected SARS-CoV-2 RNA in 12% of the corresponding wastewater samples. The results of this study suggest that sampling from decentralized wastewater infrastructure can be used for continuous monitoring of SARS-CoV-2 infections.
Keywords: SARS-CoV-2; septic tanks; wastewater-based epidemiology.