tetano
Editor, Senior Moderator
JACC Basic Transl Sci
. 2022 Sep 14.
doi: 10.1016/j.jacbts.2022.09.001. Online ahead of print.
Human Cardiac Pericytes are Susceptible to SARS-CoV-2 Infection
Brittany D Brumback[SUP] 1 2 [/SUP], Oleksandr Dmytrenko[SUP] 1 [/SUP], Ashley N Robinson[SUP] 1 [/SUP], Adam L Bailey[SUP] 3 4 [/SUP], Pan Ma[SUP] 1 [/SUP], Jing Liu[SUP] 1 [/SUP], Stephanie C Hicks[SUP] 1 [/SUP], Sherwin Ng[SUP] 1 [/SUP], Gang Li[SUP] 1 2 [/SUP], David M Zhang[SUP] 1 [/SUP], Catherine E Lipovsky[SUP] 1 5 [/SUP], Chieh-Yu Lin[SUP] 3 [/SUP], Michael S Diamond[SUP] 3 6 7 [/SUP], Kory Lavine[SUP] 1 3 5 [/SUP], Stacey L Rentschler[SUP] 1 2 5 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to upregulation of inflammatory markers, vasoactive mediators, and NF-κB-dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19.
Keywords: ACE2, angiotensin converting enzyme 2; COVID-19; COVID-19, coronavirus disease-2019; SARS-CoV-2; SARS-CoV-2, severe acute respiratory syndromecoronavirus-2; cardiovascular disease; pericytes.
. 2022 Sep 14.
doi: 10.1016/j.jacbts.2022.09.001. Online ahead of print.
Human Cardiac Pericytes are Susceptible to SARS-CoV-2 Infection
Brittany D Brumback[SUP] 1 2 [/SUP], Oleksandr Dmytrenko[SUP] 1 [/SUP], Ashley N Robinson[SUP] 1 [/SUP], Adam L Bailey[SUP] 3 4 [/SUP], Pan Ma[SUP] 1 [/SUP], Jing Liu[SUP] 1 [/SUP], Stephanie C Hicks[SUP] 1 [/SUP], Sherwin Ng[SUP] 1 [/SUP], Gang Li[SUP] 1 2 [/SUP], David M Zhang[SUP] 1 [/SUP], Catherine E Lipovsky[SUP] 1 5 [/SUP], Chieh-Yu Lin[SUP] 3 [/SUP], Michael S Diamond[SUP] 3 6 7 [/SUP], Kory Lavine[SUP] 1 3 5 [/SUP], Stacey L Rentschler[SUP] 1 2 5 [/SUP]
Affiliations
- PMID: 36124009
- PMCID: PMC9473702
- DOI: 10.1016/j.jacbts.2022.09.001
Abstract
Coronavirus disease 2019 (COVID-19) is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to upregulation of inflammatory markers, vasoactive mediators, and NF-κB-dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19.
Keywords: ACE2, angiotensin converting enzyme 2; COVID-19; COVID-19, coronavirus disease-2019; SARS-CoV-2; SARS-CoV-2, severe acute respiratory syndromecoronavirus-2; cardiovascular disease; pericytes.