tetano
Editor, Senior Moderator
Jpn J Infect Dis
. 2020 Jun 30.
doi: 10.7883/yoken.JJID.2020.324. Online ahead of print.
An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2
Kazuya Shirato[SUP] 1 [/SUP], Naganori Nao[SUP] 1 [/SUP], Shutoku Matsuyama[SUP] 1 [/SUP], Makoto Takeda[SUP] 1 [/SUP], Tsutomu Kageyama[SUP] 2 [/SUP]
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Abstract
The disease caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, in December 2019 is currently spreading rapidly worldwide. SARS-CoV-2 is usually detected via real-time RT-PCR. However, as institutions/hospitals deal with increasing numbers of specimens, a simpler detection system is required. Here, we present an ultra-rapid, real-time RT-PCR assay for SARS-CoV-2 using the PCR1100 device. Although this tests only one specimen at any one time, the amplification period is <20 min, with maintenance of the sensitivity and specificity of conventional real-time RT-PCR performed using large instruments. The method will be very helpful if SARS-CoV-2 testing is required a few times daily, for example to confirm virus-free status prior to discharge.
Keywords: PCR1100; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); ultra-rapid real-time RT-PCR.
. 2020 Jun 30.
doi: 10.7883/yoken.JJID.2020.324. Online ahead of print.
An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2
Kazuya Shirato[SUP] 1 [/SUP], Naganori Nao[SUP] 1 [/SUP], Shutoku Matsuyama[SUP] 1 [/SUP], Makoto Takeda[SUP] 1 [/SUP], Tsutomu Kageyama[SUP] 2 [/SUP]
AffiliationsExpand
- PMID: 32611983
- DOI: 10.7883/yoken.JJID.2020.324
Abstract
The disease caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, in December 2019 is currently spreading rapidly worldwide. SARS-CoV-2 is usually detected via real-time RT-PCR. However, as institutions/hospitals deal with increasing numbers of specimens, a simpler detection system is required. Here, we present an ultra-rapid, real-time RT-PCR assay for SARS-CoV-2 using the PCR1100 device. Although this tests only one specimen at any one time, the amplification period is <20 min, with maintenance of the sensitivity and specificity of conventional real-time RT-PCR performed using large instruments. The method will be very helpful if SARS-CoV-2 testing is required a few times daily, for example to confirm virus-free status prior to discharge.
Keywords: PCR1100; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); ultra-rapid real-time RT-PCR.