tetano
Editor, Senior Moderator
Jpn J Infect Dis
. 2023 Jun 30.
doi: 10.7883/yoken.JJID.2023.140. Online ahead of print. Analysis of COVID-19 in tissues acquired by minimally invasive autopsy in out-of-hospital deaths with postmortem degeneration
Yuichiro Hirata[SUP] 1 2 [/SUP], Yohsuke Makino[SUP] 2 3 [/SUP], Shun Iida[SUP] 1 [/SUP], Harutaka Katano[SUP] 1 [/SUP], Sayaka Nagasawa[SUP] 2 [/SUP], Hirofumi Rokutan[SUP] 4 [/SUP], Munetoshi Hinata[SUP] 4 [/SUP], Akiko Iwasaki[SUP] 4 [/SUP], Yoichi Yasunaga[SUP] 4 [/SUP], Hiroyuki Abe[SUP] 4 [/SUP], Masako Ikemura[SUP] 4 [/SUP], Ayumi Motomura[SUP] 2 3 5 [/SUP], Kei Kira[SUP] 2 3 [/SUP], Susumu Kobayashi[SUP] 3 [/SUP], Shigeki Tsuneya[SUP] 2 3 [/SUP], Suguru Torimitsu[SUP] 2 3 [/SUP], Isao Yamamoto[SUP] 6 [/SUP], Kimiko Nakagawa[SUP] 6 [/SUP], Iwao Hasegawa[SUP] 6 [/SUP], Shinji Akitomi[SUP] 3 7 [/SUP], Daisuke Yajima[SUP] 5 [/SUP], Tetsuo Ushiku[SUP] 4 [/SUP], Hisako Saitoh[SUP] 2 8 [/SUP], Tadaki Suzuki[SUP] 1 [/SUP], Hirotaro Iwase[SUP] 2 3 [/SUP]
Affiliations
Minimally invasive autopsy (MIA) is an alternative to full autopsy for collection of tissue samples from patient's bodies, using an instrument such as a biopsy needle. MIA has been conducted in many cases of coronavirus disease 2019 (COVID-19) and contributed to elucidation of the pathogenesis of the disease. However, most of these cases were hospital deaths, and there are few reports on the application of MIA in out-of-hospital deaths with varying extent of postmortem changes. In this study, MIA and autopsy were both performed in 15 COVID-19 cases 2-30 days after death, including 11 out-of-hospital deaths. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome detection by reverse transcriptase quantitative polymerase chain reaction using MIA samples was mostly consistent with that using autopsy samples, especially for lung tissue, even in out-of-hospital cases. MIA had high sensitivity and specificity (> 0.80). Histological examination of lung tissue obtained by MIA showed characteristics of COVID-19 pneumonia, with 91% agreement with autopsy samples, and localization of SARS-CoV-2 protein in lung tissue was indicated by immunohistochemistry, with 75% agreement. These results suggest that MIA is applicable to out-of-hospital deaths of COVID-19 with a variety of postmortem changes, especially when autopsy is not available.
Keywords: COVID-19; autopsy; minimally invasive autopsy; out-of-hospital death; postmortem change.
. 2023 Jun 30.
doi: 10.7883/yoken.JJID.2023.140. Online ahead of print. Analysis of COVID-19 in tissues acquired by minimally invasive autopsy in out-of-hospital deaths with postmortem degeneration
Yuichiro Hirata[SUP] 1 2 [/SUP], Yohsuke Makino[SUP] 2 3 [/SUP], Shun Iida[SUP] 1 [/SUP], Harutaka Katano[SUP] 1 [/SUP], Sayaka Nagasawa[SUP] 2 [/SUP], Hirofumi Rokutan[SUP] 4 [/SUP], Munetoshi Hinata[SUP] 4 [/SUP], Akiko Iwasaki[SUP] 4 [/SUP], Yoichi Yasunaga[SUP] 4 [/SUP], Hiroyuki Abe[SUP] 4 [/SUP], Masako Ikemura[SUP] 4 [/SUP], Ayumi Motomura[SUP] 2 3 5 [/SUP], Kei Kira[SUP] 2 3 [/SUP], Susumu Kobayashi[SUP] 3 [/SUP], Shigeki Tsuneya[SUP] 2 3 [/SUP], Suguru Torimitsu[SUP] 2 3 [/SUP], Isao Yamamoto[SUP] 6 [/SUP], Kimiko Nakagawa[SUP] 6 [/SUP], Iwao Hasegawa[SUP] 6 [/SUP], Shinji Akitomi[SUP] 3 7 [/SUP], Daisuke Yajima[SUP] 5 [/SUP], Tetsuo Ushiku[SUP] 4 [/SUP], Hisako Saitoh[SUP] 2 8 [/SUP], Tadaki Suzuki[SUP] 1 [/SUP], Hirotaro Iwase[SUP] 2 3 [/SUP]
Affiliations
- PMID: 37394459
- DOI: 10.7883/yoken.JJID.2023.140
Minimally invasive autopsy (MIA) is an alternative to full autopsy for collection of tissue samples from patient's bodies, using an instrument such as a biopsy needle. MIA has been conducted in many cases of coronavirus disease 2019 (COVID-19) and contributed to elucidation of the pathogenesis of the disease. However, most of these cases were hospital deaths, and there are few reports on the application of MIA in out-of-hospital deaths with varying extent of postmortem changes. In this study, MIA and autopsy were both performed in 15 COVID-19 cases 2-30 days after death, including 11 out-of-hospital deaths. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome detection by reverse transcriptase quantitative polymerase chain reaction using MIA samples was mostly consistent with that using autopsy samples, especially for lung tissue, even in out-of-hospital cases. MIA had high sensitivity and specificity (> 0.80). Histological examination of lung tissue obtained by MIA showed characteristics of COVID-19 pneumonia, with 91% agreement with autopsy samples, and localization of SARS-CoV-2 protein in lung tissue was indicated by immunohistochemistry, with 75% agreement. These results suggest that MIA is applicable to out-of-hospital deaths of COVID-19 with a variety of postmortem changes, especially when autopsy is not available.
Keywords: COVID-19; autopsy; minimally invasive autopsy; out-of-hospital death; postmortem change.