tetano
Editor, Senior Moderator
Jpn J Infect Dis
. 2022 Jul 29.
doi: 10.7883/yoken.JJID.2021.841. Online ahead of print.
Detection of SARS-CoV-2 Genome for over 100 Days after COVID-19 Onset
Keiko Goto[SUP] 1 [/SUP], Kenichi Komatsu[SUP] 2 [/SUP], Tsuyoshi Sekizuka[SUP] 3 [/SUP], Hiromi Ebisawa[SUP] 2 [/SUP], Miki Ootake[SUP] 4 [/SUP], Megumi Honda[SUP] 4 [/SUP], Noriko Nagata[SUP] 1 [/SUP], Daisuke Yoshida[SUP] 1 [/SUP], Toshikazu Yanaoka[SUP] 1 [/SUP], Hirokazu Kimura[SUP] 5 [/SUP], Makoto Kuroda[SUP] 3 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019, is spreading globally. In general, the viral genome becomes undetectable within a couple of weeks after infection. Herein, we report a case of long-term detection of the SARS-CoV-2 genome from the same individual for 106 days. Whole genome sequencing was performed on specimens taken at the onset of the disease and at 2 months after onset, and the B.1.1.7 lineage was detected in both samples. Comparison of the full-length sequences revealed a single-base difference and no amino acid mutations. This is the first case in Japan where the virus was detected over a long time, and the full-length sequences were compared.
Keywords: B.1.1.7 lineage; PCR; SARS-CoV-2; long-term detection; whole genome sequencing.
. 2022 Jul 29.
doi: 10.7883/yoken.JJID.2021.841. Online ahead of print.
Detection of SARS-CoV-2 Genome for over 100 Days after COVID-19 Onset
Keiko Goto[SUP] 1 [/SUP], Kenichi Komatsu[SUP] 2 [/SUP], Tsuyoshi Sekizuka[SUP] 3 [/SUP], Hiromi Ebisawa[SUP] 2 [/SUP], Miki Ootake[SUP] 4 [/SUP], Megumi Honda[SUP] 4 [/SUP], Noriko Nagata[SUP] 1 [/SUP], Daisuke Yoshida[SUP] 1 [/SUP], Toshikazu Yanaoka[SUP] 1 [/SUP], Hirokazu Kimura[SUP] 5 [/SUP], Makoto Kuroda[SUP] 3 [/SUP]
Affiliations
- PMID: 35908868
- DOI: 10.7883/yoken.JJID.2021.841
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019, is spreading globally. In general, the viral genome becomes undetectable within a couple of weeks after infection. Herein, we report a case of long-term detection of the SARS-CoV-2 genome from the same individual for 106 days. Whole genome sequencing was performed on specimens taken at the onset of the disease and at 2 months after onset, and the B.1.1.7 lineage was detected in both samples. Comparison of the full-length sequences revealed a single-base difference and no amino acid mutations. This is the first case in Japan where the virus was detected over a long time, and the full-length sequences were compared.
Keywords: B.1.1.7 lineage; PCR; SARS-CoV-2; long-term detection; whole genome sequencing.