tetano
Editor, Senior Moderator
Jpn J Infect Dis
. 2025 Jun 30.
doi: 10.7883/yoken.JJID.2024.196. Online ahead of print. Variability in single radial immunodiffusion (SRID) potency affected by influenza vaccine reference antigen/antiserum combinations: relationship between dissociation constant and robustness of SRID potency
Haruna Nishijima[SUP] 1 [/SUP], Noriko Shimasaki[SUP] 1 [/SUP], Tomoko Kuwahara[SUP] 1 [/SUP], Yusuke Nakai[SUP] 1 [/SUP], Kazuya Nakamura[SUP] 2 [/SUP], Kayoko Sato[SUP] 1 [/SUP], Keiko Murano[SUP] 1 [/SUP], Shigeyuki Itamura[SUP] 2 [/SUP], Akihide Ryo[SUP] 1 [/SUP], Yuichi Harada[SUP] 1 [/SUP]
Affiliations
The potencies of domestic influenza virus reference antigens were initially calibrated using a single radial immunodiffusion (SRID) assay using primarily prepared international reference antigens. The SRID potency should not be affected when using another reference antigen calibrated with the same international antigen. However, the SRID potency of the test antigens can vary, although the causes of these discrepancies remain unclear. Here, we calibrated two candidate reference antigens (LotA and LotB) in the A(H3N2) subtype with various pairs of reference reagent sets (antigen and antiserum). The potencies of LotA and LotB varied depending on the reagent pair used, with a more pronounced effect in LotA (CV = 5.4% vs. 3.8%). To explore the cause of these divergences, we analyzed the dissociation constant of each reagent pair and scored them based on the hypothesis that pairs exhibiting stronger antigen-antibody binding would have smaller precipitin rings. Comparing these scores with the respective potency scores, we observed a strong correlation between the Binding score (relative BLI-K[SUB]D[/SUB]) and potency score in LotA (r = 0.8464, p = 0.0001) but not in LotB (r = 0.4000, p = 0.1408). These data suggest that antigen-antibody binding strength is an influencing factor of SRID potency.
Keywords: SRID potency; dissociation constant; reference reagent; seasonal influenza vaccine; single radial immunodiffusion assay (SRID).
. 2025 Jun 30.
doi: 10.7883/yoken.JJID.2024.196. Online ahead of print. Variability in single radial immunodiffusion (SRID) potency affected by influenza vaccine reference antigen/antiserum combinations: relationship between dissociation constant and robustness of SRID potency
Haruna Nishijima[SUP] 1 [/SUP], Noriko Shimasaki[SUP] 1 [/SUP], Tomoko Kuwahara[SUP] 1 [/SUP], Yusuke Nakai[SUP] 1 [/SUP], Kazuya Nakamura[SUP] 2 [/SUP], Kayoko Sato[SUP] 1 [/SUP], Keiko Murano[SUP] 1 [/SUP], Shigeyuki Itamura[SUP] 2 [/SUP], Akihide Ryo[SUP] 1 [/SUP], Yuichi Harada[SUP] 1 [/SUP]
Affiliations
- PMID: 40582887
- DOI: 10.7883/yoken.JJID.2024.196
The potencies of domestic influenza virus reference antigens were initially calibrated using a single radial immunodiffusion (SRID) assay using primarily prepared international reference antigens. The SRID potency should not be affected when using another reference antigen calibrated with the same international antigen. However, the SRID potency of the test antigens can vary, although the causes of these discrepancies remain unclear. Here, we calibrated two candidate reference antigens (LotA and LotB) in the A(H3N2) subtype with various pairs of reference reagent sets (antigen and antiserum). The potencies of LotA and LotB varied depending on the reagent pair used, with a more pronounced effect in LotA (CV = 5.4% vs. 3.8%). To explore the cause of these divergences, we analyzed the dissociation constant of each reagent pair and scored them based on the hypothesis that pairs exhibiting stronger antigen-antibody binding would have smaller precipitin rings. Comparing these scores with the respective potency scores, we observed a strong correlation between the Binding score (relative BLI-K[SUB]D[/SUB]) and potency score in LotA (r = 0.8464, p = 0.0001) but not in LotB (r = 0.4000, p = 0.1408). These data suggest that antigen-antibody binding strength is an influencing factor of SRID potency.
Keywords: SRID potency; dissociation constant; reference reagent; seasonal influenza vaccine; single radial immunodiffusion assay (SRID).