tetano
Editor, Senior Moderator
J Wildl Dis. 2019 Sep 26. [Epub ahead of print]
[h=1]LIMITED DETECTION OF ANTIBODIES TO CLADE 2.3.4.4 A/GOOSE/GUANGDONG/1/1996 LINEAGE HIGHLY PATHOGENIC H5 AVIAN INFLUENZA VIRUS IN NORTH AMERICAN WATERFOWL.[/h] Stallknecht DE[SUP]1[/SUP], Kienzle-Dean C[SUP]1[/SUP], Davis-Fields N[SUP]1[/SUP], Jennelle CS[SUP]2[/SUP], Bowman AS[SUP]3[/SUP], Nolting JM[SUP]3[/SUP], Boyce WM[SUP]4[/SUP], Crum JM[SUP]5[/SUP], Santos JJS[SUP]6[/SUP], Brown JD[SUP]7[/SUP], Prosser DJ[SUP]8[/SUP], De La Cruz SEW[SUP]9[/SUP], Ackerman JT[SUP]10[/SUP], Casazza ML[SUP]10[/SUP], Krauss S[SUP]11[/SUP], Perez DR[SUP]6[/SUP], Ramey AM[SUP]12[/SUP], Poulson RL[SUP]1[/SUP].
[h=3]Author information[/h] 1 Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D. W. Brooks Drive, Athens, Georgia 30602, USA. 2 Wildlife Health Program, Minnesota Department of Natural Resources, 5463 West Broadway Avenue, Forest Lake, Minnesota 55025, USA. 3 Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, Ohio 43210, USA. 4 School of Veterinary Medicine, Wildlife Health Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA. 5 Wildlife Resources Section, West Virginia Division of Natural Resources, Elkins, West Virginia 26241, USA. 6 Poultry Disease Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA. 7 Animal Diagnostic Laboratory, Pennsylvania Game Commission, Wiley Lane, University Park, Pennsylvania 16801, USA. 8 Patuxent Wildlife Research Center, Beltsville Laboratory, US Geological Survey, 12100 Beech Forest Road, Laurel, Maryland 20708, USA. 9 Western Ecological Research Center, San Francisco Bay Estuary Field Station, US Geological Survey, 505 Azuar Drive, Vallejo, California 94592, USA. 10 Western Ecological Research Center, Dixon Field Station, US Geological Survey, 800 Business Park Drive, Suite D, Dixon, California 95620, USA. 11 Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA. 12 Alaska Science Center, US Geological Survey, 4210 University Drive, Anchorage, Alaska 99508, USA.
[h=3]Abstract[/h] During 2014, highly pathogenic (HP) influenza A viruses (IAVs) of the A/Goose/Guangdong/1/1996 lineage (GsGD-HP-H5), originating from Asia, were detected in domestic poultry and wild birds in Canada and the US. These clade 2.3.4.4 GsGD-HP-H5 viruses included reassortants possessing North American lineage gene segments; were detected in wild birds in the Pacific, Central, and Mississippi flyways; and caused the largest HP IAV outbreak in poultry in US history. To determine if an antibody response indicative of previous infection with clade 2.3.4.4 GsGD-HP-H5 IAV could be detected in North American wild waterfowl sampled before, during, and after the 2014-15 outbreak, sera from 2,793 geese and 3,715 ducks were tested by blocking enzyme-linked immunosorbent assay and hemagglutination inhibition (HI) tests using both clade 2.3.4.4 GsGD-HP-H5 and North American lineage low pathogenic (LP) H5 IAV antigens. We detected an antibody response meeting a comparative titer-based criteria (HI titer observed with 2.3.4.4 GsGD-HP-H5 antigens exceeded the titer observed for LP H5 antigen by two or more dilutions) for previous infection with clade 2.3.4.4 GsGD-HP-H5 IAV in only five birds, one Blue-winged Teal (Spatula discors) sampled during the outbreak and three Mallards (Anas platyrhynchos) and one Canada Goose (Branta canadensis) sampled during the post-outbreak period. These serologic results are consistent with the spatiotemporal extent of the outbreak in wild birds in North America during 2014 and 2015 and limited exposure of waterfowl to GsGD-HP-H5 IAV, particularly in the central and eastern US.
[h=4]KEYWORDS:[/h] Clade 2.3.4.4; H5; North America; ducks; geese; hemagglutination inhibition; influenza A virus; serology
PMID: 31556839
[h=1]LIMITED DETECTION OF ANTIBODIES TO CLADE 2.3.4.4 A/GOOSE/GUANGDONG/1/1996 LINEAGE HIGHLY PATHOGENIC H5 AVIAN INFLUENZA VIRUS IN NORTH AMERICAN WATERFOWL.[/h] Stallknecht DE[SUP]1[/SUP], Kienzle-Dean C[SUP]1[/SUP], Davis-Fields N[SUP]1[/SUP], Jennelle CS[SUP]2[/SUP], Bowman AS[SUP]3[/SUP], Nolting JM[SUP]3[/SUP], Boyce WM[SUP]4[/SUP], Crum JM[SUP]5[/SUP], Santos JJS[SUP]6[/SUP], Brown JD[SUP]7[/SUP], Prosser DJ[SUP]8[/SUP], De La Cruz SEW[SUP]9[/SUP], Ackerman JT[SUP]10[/SUP], Casazza ML[SUP]10[/SUP], Krauss S[SUP]11[/SUP], Perez DR[SUP]6[/SUP], Ramey AM[SUP]12[/SUP], Poulson RL[SUP]1[/SUP].
[h=3]Author information[/h] 1 Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, 589 D. W. Brooks Drive, Athens, Georgia 30602, USA. 2 Wildlife Health Program, Minnesota Department of Natural Resources, 5463 West Broadway Avenue, Forest Lake, Minnesota 55025, USA. 3 Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, Ohio 43210, USA. 4 School of Veterinary Medicine, Wildlife Health Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA. 5 Wildlife Resources Section, West Virginia Division of Natural Resources, Elkins, West Virginia 26241, USA. 6 Poultry Disease Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA. 7 Animal Diagnostic Laboratory, Pennsylvania Game Commission, Wiley Lane, University Park, Pennsylvania 16801, USA. 8 Patuxent Wildlife Research Center, Beltsville Laboratory, US Geological Survey, 12100 Beech Forest Road, Laurel, Maryland 20708, USA. 9 Western Ecological Research Center, San Francisco Bay Estuary Field Station, US Geological Survey, 505 Azuar Drive, Vallejo, California 94592, USA. 10 Western Ecological Research Center, Dixon Field Station, US Geological Survey, 800 Business Park Drive, Suite D, Dixon, California 95620, USA. 11 Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA. 12 Alaska Science Center, US Geological Survey, 4210 University Drive, Anchorage, Alaska 99508, USA.
[h=3]Abstract[/h] During 2014, highly pathogenic (HP) influenza A viruses (IAVs) of the A/Goose/Guangdong/1/1996 lineage (GsGD-HP-H5), originating from Asia, were detected in domestic poultry and wild birds in Canada and the US. These clade 2.3.4.4 GsGD-HP-H5 viruses included reassortants possessing North American lineage gene segments; were detected in wild birds in the Pacific, Central, and Mississippi flyways; and caused the largest HP IAV outbreak in poultry in US history. To determine if an antibody response indicative of previous infection with clade 2.3.4.4 GsGD-HP-H5 IAV could be detected in North American wild waterfowl sampled before, during, and after the 2014-15 outbreak, sera from 2,793 geese and 3,715 ducks were tested by blocking enzyme-linked immunosorbent assay and hemagglutination inhibition (HI) tests using both clade 2.3.4.4 GsGD-HP-H5 and North American lineage low pathogenic (LP) H5 IAV antigens. We detected an antibody response meeting a comparative titer-based criteria (HI titer observed with 2.3.4.4 GsGD-HP-H5 antigens exceeded the titer observed for LP H5 antigen by two or more dilutions) for previous infection with clade 2.3.4.4 GsGD-HP-H5 IAV in only five birds, one Blue-winged Teal (Spatula discors) sampled during the outbreak and three Mallards (Anas platyrhynchos) and one Canada Goose (Branta canadensis) sampled during the post-outbreak period. These serologic results are consistent with the spatiotemporal extent of the outbreak in wild birds in North America during 2014 and 2015 and limited exposure of waterfowl to GsGD-HP-H5 IAV, particularly in the central and eastern US.
[h=4]KEYWORDS:[/h] Clade 2.3.4.4; H5; North America; ducks; geese; hemagglutination inhibition; influenza A virus; serology
PMID: 31556839