tetano
Editor, Senior Moderator
Immunol Res. 2012 Sep 30. [Epub ahead of print]
Limited replication of influenza A virus in human mast cells.
Marcet CW, St Laurent CD, Moon TC, Singh N, Befus AD.
Source
Pulmonary Research Group, Department of Medicine, Room 567, HMRC, University of Alberta, Edmonton, AB, T6G 2S2, Canada.
Abstract
Mast cells are important in innate immunity and protective against certain bacterial infections. However, there is limited evidence that mast cells respond to viruses. As mast cells are abundant in mucosal tissues of the lung, they are in a prime location to detect and respond to influenza virus. In this study, we characterized for the first time the replication cycle of influenza A virus in human mast cells by measuring influenza A virus transcription, RNA replication, protein synthesis, and formation of infectious virus as compared to the replication cycle in epithelial cells. We detected the presence of influenza A viral genomic RNA transcription, replication, and protein synthesis in human mast cells and epithelial cells. However, there was no significant release of infectious influenza A virus from mast cells, whereas epithelial cells produce ~100-fold virus compared with the inoculating dose. We confirmed that influenza A virus infects human mast cells, begins to replicate, but the production of new virus is aborted. Thus, mast cells may lack critical factors essential for productive infection or there are intrinsic or inducible anti-influenza A mechanisms in mast cells.
PMID:
23055084
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23055084
Limited replication of influenza A virus in human mast cells.
Marcet CW, St Laurent CD, Moon TC, Singh N, Befus AD.
Source
Pulmonary Research Group, Department of Medicine, Room 567, HMRC, University of Alberta, Edmonton, AB, T6G 2S2, Canada.
Abstract
Mast cells are important in innate immunity and protective against certain bacterial infections. However, there is limited evidence that mast cells respond to viruses. As mast cells are abundant in mucosal tissues of the lung, they are in a prime location to detect and respond to influenza virus. In this study, we characterized for the first time the replication cycle of influenza A virus in human mast cells by measuring influenza A virus transcription, RNA replication, protein synthesis, and formation of infectious virus as compared to the replication cycle in epithelial cells. We detected the presence of influenza A viral genomic RNA transcription, replication, and protein synthesis in human mast cells and epithelial cells. However, there was no significant release of infectious influenza A virus from mast cells, whereas epithelial cells produce ~100-fold virus compared with the inoculating dose. We confirmed that influenza A virus infects human mast cells, begins to replicate, but the production of new virus is aborted. Thus, mast cells may lack critical factors essential for productive infection or there are intrinsic or inducible anti-influenza A mechanisms in mast cells.
PMID:
23055084
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23055084