tetano
Editor, Senior Moderator
Mol Cell. 2014 Feb 6;53(3):393-406. doi: 10.1016/j.molcel.2014.01.009.
Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli.
Imamura K1, Imamachi N2, Akizuki G2, Kumakura M3, Kawaguchi A3, Nagata K3, Kato A4, Kawaguchi Y4, Sato H5, Yoneda M5, Kai C5, Yada T6, Suzuki Y7, Yamada T8, Ozawa T8, Kaneki K9, Inoue T9, Kobayashi M9, Kodama T9, Wada Y10, Sekimizu K1, Akimitsu N11.
Author information
Abstract
Although thousands of long noncoding RNAs (lncRNAs) are localized in the nucleus, only a few dozen have been functionally characterized. Here we show that nuclear enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, is induced by influenza virus and herpes simplex virus infection as well as by Toll-like receptor3-p38 pathway-triggered poly I:C stimulation, resulting in excess formation of paraspeckles. We found that NEAT1 facilitates the expression of antiviral genes including cytokines such as interleukin-8 (IL8). We found that splicing factor proline/glutamine-rich (SFPQ), a NEAT1-binding paraspeckle protein, is a repressor of IL8 transcription, and that NEAT1 induction relocates SFPQ from the IL8 promoter to the paraspeckles, leading to transcriptional activation of IL8. Together, our data show that NEAT1 plays an important role in the innate immune response through the transcriptional regulation of antiviral genes by the stimulus-responsive cooperative action of NEAT1 and SFPQ.
Copyright ? 2014 Elsevier Inc. All rights reserved.
PMID:
24507715
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24507715
Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli.
Imamura K1, Imamachi N2, Akizuki G2, Kumakura M3, Kawaguchi A3, Nagata K3, Kato A4, Kawaguchi Y4, Sato H5, Yoneda M5, Kai C5, Yada T6, Suzuki Y7, Yamada T8, Ozawa T8, Kaneki K9, Inoue T9, Kobayashi M9, Kodama T9, Wada Y10, Sekimizu K1, Akimitsu N11.
Author information
Abstract
Although thousands of long noncoding RNAs (lncRNAs) are localized in the nucleus, only a few dozen have been functionally characterized. Here we show that nuclear enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, is induced by influenza virus and herpes simplex virus infection as well as by Toll-like receptor3-p38 pathway-triggered poly I:C stimulation, resulting in excess formation of paraspeckles. We found that NEAT1 facilitates the expression of antiviral genes including cytokines such as interleukin-8 (IL8). We found that splicing factor proline/glutamine-rich (SFPQ), a NEAT1-binding paraspeckle protein, is a repressor of IL8 transcription, and that NEAT1 induction relocates SFPQ from the IL8 promoter to the paraspeckles, leading to transcriptional activation of IL8. Together, our data show that NEAT1 plays an important role in the innate immune response through the transcriptional regulation of antiviral genes by the stimulus-responsive cooperative action of NEAT1 and SFPQ.
Copyright ? 2014 Elsevier Inc. All rights reserved.
PMID:
24507715
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24507715