tetano
Editor, Senior Moderator
ACS Appl Mater Interfaces. 2018 Mar 29. doi: 10.1021/acsami.8b02735. [Epub ahead of print]
[h=1]Magnetic nanozyme-linked immunosorbent assay for ultrasensitive influenza A virus detection.[/h] Oh S, Kim J, Tran VT, Lee DK, Ahmed SR, Hong JC, Lee J, Park EY, Lee J.
[h=3]Abstract[/h] Rapid and sensitive detection of influenza virus is of soaring importance to prevent further spread of viruses and adequate clinical treatment. Herein, an ultra-sensitive colorimetric assay, called magnetic nano(e)zyme - linked immunosorbent assay (MagLISA) is suggested, in which silica-shelled magnetic nanobeads (MagNBs) and gold nanoparticles are combined to monitor influenza A virus up to femtogram per milliliter concentration. Two essential strategies for ultrasensitive sensing are designed i.e., facile target separation by MagNBs and signal amplification by the enzyme-like activity of gold nanozymes (AuNZs). The enzyme-like activity was experimentally and computationally evaluated, where the catalicity of AuNZ was tremendously stronger than typical biological enzymes. In the spiked test, a straightforward linearity was presented in the range of 5.0 ? 10[SUP]-15 ~ -6[/SUP]g/mL, detecting influenza virus A (New Caledonia/20/1999) (H1N1) only by human eyes (up to 5.0 ? 10[SUP]-12[/SUP] g/mL) as well as a microplate reader (up to 44.2 ? 10[SUP]-15[/SUP] g/mL), which is the lowest record to monitor influenza virus using ELISA based technology as far as we know. Clinically isolate human serum samples were successfully monitored at the detection limit of 2.6 PFU/mL. This novel MagLISA demonstrates, therefore, a puissant sensing platform possessing the advances of fathomable sample separation, enrichment, ultra-sensitive readout, and anti-interference ability may reduce the spread of influenza virus and provide immediate clinical treatment.
PMID: 29595253 DOI: 10.1021/acsami.8b02735
[h=1]Magnetic nanozyme-linked immunosorbent assay for ultrasensitive influenza A virus detection.[/h] Oh S, Kim J, Tran VT, Lee DK, Ahmed SR, Hong JC, Lee J, Park EY, Lee J.
[h=3]Abstract[/h] Rapid and sensitive detection of influenza virus is of soaring importance to prevent further spread of viruses and adequate clinical treatment. Herein, an ultra-sensitive colorimetric assay, called magnetic nano(e)zyme - linked immunosorbent assay (MagLISA) is suggested, in which silica-shelled magnetic nanobeads (MagNBs) and gold nanoparticles are combined to monitor influenza A virus up to femtogram per milliliter concentration. Two essential strategies for ultrasensitive sensing are designed i.e., facile target separation by MagNBs and signal amplification by the enzyme-like activity of gold nanozymes (AuNZs). The enzyme-like activity was experimentally and computationally evaluated, where the catalicity of AuNZ was tremendously stronger than typical biological enzymes. In the spiked test, a straightforward linearity was presented in the range of 5.0 ? 10[SUP]-15 ~ -6[/SUP]g/mL, detecting influenza virus A (New Caledonia/20/1999) (H1N1) only by human eyes (up to 5.0 ? 10[SUP]-12[/SUP] g/mL) as well as a microplate reader (up to 44.2 ? 10[SUP]-15[/SUP] g/mL), which is the lowest record to monitor influenza virus using ELISA based technology as far as we know. Clinically isolate human serum samples were successfully monitored at the detection limit of 2.6 PFU/mL. This novel MagLISA demonstrates, therefore, a puissant sensing platform possessing the advances of fathomable sample separation, enrichment, ultra-sensitive readout, and anti-interference ability may reduce the spread of influenza virus and provide immediate clinical treatment.
PMID: 29595253 DOI: 10.1021/acsami.8b02735