Laidback Al
Well-known member
doi: 10.1128/mBio.00180-12 <cite> 14 August 2012 <abbr title="mBio" class="slug-jnl-abbrev"> mBio</abbr> vol. 3 no. 4 e00180-12 </cite>
Identification, Characterization, and In Vitro Culture of Highly Divergent Arenaviruses from Boa Constrictors and Annulated Tree Boas: Candidate Etiological Agents for Snake Inclusion Body Disease
Mark D. Stenglein<sup>a</sup>, Chris Sanders<sup>b</sup>, Amy L. Kistler<sup>a</sup>*, J. Graham Ruby<sup>a</sup>, Jessica Y. Franco<sup>a</sup>, Drury R. Reavill<sup>c</sup>, Freeland Dunker<sup>d</sup>, and Joseph L. DeRisi<sup>a,e</sup>
ABSTRACT
Inclusion body disease (IBD) is an infectious fatal disease of snakes typified by behavioral abnormalities, wasting, and secondary infections. At a histopathological level, the disease is identified by the presence of large eosinophilic cytoplasmic inclusions in multiple tissues. To date, no virus or other pathogen has been definitively characterized or associated with the disease. Using a metagenomic approach to search for candidate etiologic agents in snakes with confirmed IBD, we identified and de novo assembled the complete genomic sequences of two viruses related to arenaviruses, and a third arenavirus-like sequence was discovered by screening an additional set of samples. A continuous boa constrictor cell line was established and used to propagate and isolate one of the viruses in culture. Viral nucleoprotein was localized and concentrated within large cytoplasmic inclusions in infected cells in culture and tissues from diseased snakes. In total, viral RNA was detected in 6/8 confirmed IBD cases and 0/18 controls. These viruses have a typical arenavirus genome organization but are highly divergent, belonging to a lineage separate from that of the Old and New World arenaviruses. Furthermore, these viruses encode envelope glycoproteins that are more similar to those of filoviruses than to those of other arenaviruses. These findings implicate these viruses as candidate etiologic agents of IBD. The presence of arenaviruses outside mammals reveals that these viruses infect an unexpectedly broad range of species and represent a new reservoir of potential human pathogens.
http://mbio.asm.org/content/3/4/e00180-12.abstract?sid=2117273a-34f6-4dc5-8691-0b8a9df0e140
full open access text here: http://mbio.asm.org/content/3/4/e00180-12.full?sid=2117273a-34f6-4dc5-8691-0b8a9df0e140
hat tip alert
Identification, Characterization, and In Vitro Culture of Highly Divergent Arenaviruses from Boa Constrictors and Annulated Tree Boas: Candidate Etiological Agents for Snake Inclusion Body Disease
Mark D. Stenglein<sup>a</sup>, Chris Sanders<sup>b</sup>, Amy L. Kistler<sup>a</sup>*, J. Graham Ruby<sup>a</sup>, Jessica Y. Franco<sup>a</sup>, Drury R. Reavill<sup>c</sup>, Freeland Dunker<sup>d</sup>, and Joseph L. DeRisi<sup>a,e</sup>
ABSTRACT
Inclusion body disease (IBD) is an infectious fatal disease of snakes typified by behavioral abnormalities, wasting, and secondary infections. At a histopathological level, the disease is identified by the presence of large eosinophilic cytoplasmic inclusions in multiple tissues. To date, no virus or other pathogen has been definitively characterized or associated with the disease. Using a metagenomic approach to search for candidate etiologic agents in snakes with confirmed IBD, we identified and de novo assembled the complete genomic sequences of two viruses related to arenaviruses, and a third arenavirus-like sequence was discovered by screening an additional set of samples. A continuous boa constrictor cell line was established and used to propagate and isolate one of the viruses in culture. Viral nucleoprotein was localized and concentrated within large cytoplasmic inclusions in infected cells in culture and tissues from diseased snakes. In total, viral RNA was detected in 6/8 confirmed IBD cases and 0/18 controls. These viruses have a typical arenavirus genome organization but are highly divergent, belonging to a lineage separate from that of the Old and New World arenaviruses. Furthermore, these viruses encode envelope glycoproteins that are more similar to those of filoviruses than to those of other arenaviruses. These findings implicate these viruses as candidate etiologic agents of IBD. The presence of arenaviruses outside mammals reveals that these viruses infect an unexpectedly broad range of species and represent a new reservoir of potential human pathogens.
http://mbio.asm.org/content/3/4/e00180-12.abstract?sid=2117273a-34f6-4dc5-8691-0b8a9df0e140
full open access text here: http://mbio.asm.org/content/3/4/e00180-12.full?sid=2117273a-34f6-4dc5-8691-0b8a9df0e140
hat tip alert