tetano
Editor, Senior Moderator
J Microbiol. 2016 Oct;54(10):695-700. doi: 10.1007/s12275-016-6383-2. Epub 2016 Sep 30.
[h=1]MDA7/IL-24 is an anti-viral factor that inhibits influenza virus replication.[/h] Seong RK[SUP]1[/SUP], Choi YK[SUP]2[/SUP], Shin OS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus infection are yet to be determined. In this study, we explored the anti-viral effect of recombinant IL-24 treatment during influenza infection. Infection of human lung adenocarcinoma cells (A549) with the influenza A virus up-regulated IL-24 mRNA and protein expression in a time-dependent manner. Pre-treatment of A549 cells with recombinant IL-24 protein effectively suppressed viral plaque formation. Furthermore, IL-24 treatment of A549 cells reduced viral non-structural protein 1 (NS1) synthesis, whereas IL-24 knockdown resulted in increased viral replication. Interestingly, IL-24 treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling. Taken together, our results suggest that IL-24 exerts a potent suppressive effect on influenza viral replication and can be used in the treatment of influenza infection.
[h=4]KEYWORDS:[/h] IL-24; antiviral activities; influenza
PMID: 27687232 DOI: 10.1007/s12275-016-6383-2
[PubMed - in process]
[h=1]MDA7/IL-24 is an anti-viral factor that inhibits influenza virus replication.[/h] Seong RK[SUP]1[/SUP], Choi YK[SUP]2[/SUP], Shin OS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus infection are yet to be determined. In this study, we explored the anti-viral effect of recombinant IL-24 treatment during influenza infection. Infection of human lung adenocarcinoma cells (A549) with the influenza A virus up-regulated IL-24 mRNA and protein expression in a time-dependent manner. Pre-treatment of A549 cells with recombinant IL-24 protein effectively suppressed viral plaque formation. Furthermore, IL-24 treatment of A549 cells reduced viral non-structural protein 1 (NS1) synthesis, whereas IL-24 knockdown resulted in increased viral replication. Interestingly, IL-24 treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling. Taken together, our results suggest that IL-24 exerts a potent suppressive effect on influenza viral replication and can be used in the treatment of influenza infection.
[h=4]KEYWORDS:[/h] IL-24; antiviral activities; influenza
PMID: 27687232 DOI: 10.1007/s12275-016-6383-2
[PubMed - in process]