tetano
Editor, Senior Moderator
Med (N Y)
. 2021 Aug 14.
doi: 10.1016/j.medj.2021.08.001. Online ahead of print.
Favourable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases
Claire T Deakin[SUP] 1 2 3 4 [/SUP], Georgina H Cornish[SUP] 5 [/SUP], Kevin W Ng[SUP] 5 [/SUP], Nikhil Faulkner[SUP] 5 [/SUP], William Bolland[SUP] 5 [/SUP], Joshua Hope[SUP] 6 [/SUP], Annachiara Rosa[SUP] 6 [/SUP], Ruth Harvey[SUP] 7 [/SUP], Saira Hussain[SUP] 7 [/SUP], Christopher Earl[SUP] 8 [/SUP], Bethany R Jebson[SUP] 1 2 3 [/SUP], Meredyth G Ll Wilkinson[SUP] 1 2 3 [/SUP], Lucy R Marshall[SUP] 1 2 3 [/SUP], Kathryn O'Brien[SUP] 1 2 3 [/SUP], Elizabeth C Rosser[SUP] 1 9 [/SUP], Anna Radziszewska[SUP] 1 9 [/SUP], Hannah Peckham[SUP] 1 9 [/SUP], Harsita Patel[SUP] 10 [/SUP], Judith Heaney[SUP] 11 [/SUP], Hannah Rickman[SUP] 11 [/SUP], Stavroula Paraskevopoulou[SUP] 11 [/SUP], Catherine F Houlihan[SUP] 11 12 [/SUP], Moira J Spyer[SUP] 11 13 [/SUP], Steve J Gamblin[SUP] 14 [/SUP], John McCauley[SUP] 7 [/SUP], Eleni Nastouli[SUP] 11 13 [/SUP], Michael Levin[SUP] 10 [/SUP], Peter Cherepanov[SUP] 6 15 [/SUP], Coziana Ciurtin[SUP] 1 [/SUP], Lucy R Wedderburn[SUP] 1 2 3 [/SUP], George Kassiotis[SUP] 5 15 [/SUP]
Affiliations
Abstract
Background: Differences in humoral immunity to coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), between children and adults remain unexplained and the impact of underlying immune dysfunction or suppression unknown. Here, we sought to examine the antibody immune competence of children and adolescents with prevalent inflammatory rheumatic diseases, juvenile idiopathic arthritis (JIA), juvenile dermatomyositis (JDM) and juvenile systemic lupus erythematosus (JSLE), against the seasonal human coronavirus (HCoV)-OC43 that frequently infects this age group.
Methods: Sera were collected from JIA (n=118), JDM (n=49) and JSLE (n=30) patients, and from healthy control (n=54) children and adolescents, prior to the coronavirus disease-19 (COVID-19) pandemic. We employed sensitive flow cytometry-based assays to determine titres of antibodies that reacted with the spike and nucleoprotein of HCoV-OC43 and cross-reacted with the spike and nucleoprotein of SARS-CoV-2, and compared with respective titres in sera from patients with multisystem inflammatory syndrome in children and adolescents (MIS-C).
Findings: Despite immune dysfunction and immunosuppressive treatment, JIA, JDM and JSLE patients maintained comparable or stronger humoral responses than healthier peers, dominated by IgG antibodies to HCoV-OC43 spike, and harboured IgG antibodies that cross-reacted with SARS-CoV-2 spike. In contrast, responses to HCoV-OC43 and SARS-CoV-2 nucleoproteins exhibited delayed age-dependent class-switching and were not elevated in JIA, JDM and JSLE patients, arguing against increased exposure.
Conclusions: Consequently, autoimmune rheumatic diseases and their treatment were associated with a favourable ratio of spike to nucleoprotein antibodies.
. 2021 Aug 14.
doi: 10.1016/j.medj.2021.08.001. Online ahead of print.
Favourable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases
Claire T Deakin[SUP] 1 2 3 4 [/SUP], Georgina H Cornish[SUP] 5 [/SUP], Kevin W Ng[SUP] 5 [/SUP], Nikhil Faulkner[SUP] 5 [/SUP], William Bolland[SUP] 5 [/SUP], Joshua Hope[SUP] 6 [/SUP], Annachiara Rosa[SUP] 6 [/SUP], Ruth Harvey[SUP] 7 [/SUP], Saira Hussain[SUP] 7 [/SUP], Christopher Earl[SUP] 8 [/SUP], Bethany R Jebson[SUP] 1 2 3 [/SUP], Meredyth G Ll Wilkinson[SUP] 1 2 3 [/SUP], Lucy R Marshall[SUP] 1 2 3 [/SUP], Kathryn O'Brien[SUP] 1 2 3 [/SUP], Elizabeth C Rosser[SUP] 1 9 [/SUP], Anna Radziszewska[SUP] 1 9 [/SUP], Hannah Peckham[SUP] 1 9 [/SUP], Harsita Patel[SUP] 10 [/SUP], Judith Heaney[SUP] 11 [/SUP], Hannah Rickman[SUP] 11 [/SUP], Stavroula Paraskevopoulou[SUP] 11 [/SUP], Catherine F Houlihan[SUP] 11 12 [/SUP], Moira J Spyer[SUP] 11 13 [/SUP], Steve J Gamblin[SUP] 14 [/SUP], John McCauley[SUP] 7 [/SUP], Eleni Nastouli[SUP] 11 13 [/SUP], Michael Levin[SUP] 10 [/SUP], Peter Cherepanov[SUP] 6 15 [/SUP], Coziana Ciurtin[SUP] 1 [/SUP], Lucy R Wedderburn[SUP] 1 2 3 [/SUP], George Kassiotis[SUP] 5 15 [/SUP]
Affiliations
- PMID: 34414384
- PMCID: PMC8363467
- DOI: 10.1016/j.medj.2021.08.001
Abstract
Background: Differences in humoral immunity to coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), between children and adults remain unexplained and the impact of underlying immune dysfunction or suppression unknown. Here, we sought to examine the antibody immune competence of children and adolescents with prevalent inflammatory rheumatic diseases, juvenile idiopathic arthritis (JIA), juvenile dermatomyositis (JDM) and juvenile systemic lupus erythematosus (JSLE), against the seasonal human coronavirus (HCoV)-OC43 that frequently infects this age group.
Methods: Sera were collected from JIA (n=118), JDM (n=49) and JSLE (n=30) patients, and from healthy control (n=54) children and adolescents, prior to the coronavirus disease-19 (COVID-19) pandemic. We employed sensitive flow cytometry-based assays to determine titres of antibodies that reacted with the spike and nucleoprotein of HCoV-OC43 and cross-reacted with the spike and nucleoprotein of SARS-CoV-2, and compared with respective titres in sera from patients with multisystem inflammatory syndrome in children and adolescents (MIS-C).
Findings: Despite immune dysfunction and immunosuppressive treatment, JIA, JDM and JSLE patients maintained comparable or stronger humoral responses than healthier peers, dominated by IgG antibodies to HCoV-OC43 spike, and harboured IgG antibodies that cross-reacted with SARS-CoV-2 spike. In contrast, responses to HCoV-OC43 and SARS-CoV-2 nucleoproteins exhibited delayed age-dependent class-switching and were not elevated in JIA, JDM and JSLE patients, arguing against increased exposure.
Conclusions: Consequently, autoimmune rheumatic diseases and their treatment were associated with a favourable ratio of spike to nucleoprotein antibodies.