Meta- and Orthogonal Integration of Influenza "OMICs" Data Defines a Role for UBR4 in Virus Budding

[tetano]

Cell Host Microbe. 2015 Dec 9;18(6):723-35. doi: 10.1016/j.chom.2015.11.002.
[h=1]Meta- and Orthogonal Integration of Influenza "OMICs" Data Defines a Role for UBR4 in Virus Budding.[/h] Tripathi S[SUP]1[/SUP], Pohl MO[SUP]2[/SUP], Zhou Y[SUP]3[/SUP], Rodriguez-Frandsen A[SUP]4[/SUP], Wang G[SUP]5[/SUP], Stein DA[SUP]6[/SUP], Moulton HM[SUP]6[/SUP], DeJesus P[SUP]4[/SUP], Che J[SUP]3[/SUP], Mulder LC[SUP]5[/SUP], Y?ng?ez E[SUP]2[/SUP], Andenmatten D[SUP]2[/SUP], Pache L[SUP]4[/SUP], Manicassamy B[SUP]5[/SUP], Albrecht RA[SUP]5[/SUP], Gonzalez MG[SUP]5[/SUP], Nguyen Q[SUP]4[/SUP], Brass A[SUP]7[/SUP], Elledge S[SUP]8[/SUP], White M[SUP]9[/SUP], Shapira S[SUP]10[/SUP], Hacohen N[SUP]11[/SUP], Karlas A[SUP]12[/SUP], Meyer TF[SUP]12[/SUP], Shales M[SUP]13[/SUP], Gatorano A[SUP]4[/SUP], Johnson JR[SUP]13[/SUP], Jang G[SUP]13[/SUP], Johnson T[SUP]13[/SUP], Verschueren E[SUP]13[/SUP], Sanders D[SUP]13[/SUP], Krogan N[SUP]13[/SUP], Shaw M[SUP]5[/SUP], K?nig R[SUP]14[/SUP], Stertz S[SUP]15[/SUP], Garc?a-Sastre A[SUP]16[/SUP], Chanda SK[SUP]17[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] Several systems-level datasets designed to dissect host-pathogen interactions during influenza A infection have been reported. However, apparent discordance among these data has hampered their full utility toward advancing mechanistic and therapeutic knowledge. To collectively reconcile these datasets, we performed a meta-analysis of data from eight published RNAi screens and integrated these data with three protein interaction datasets, including one generated within the context of this study. Further integration of these data with global virus-host interaction analyses revealed a functionally validated biochemical landscape of the influenza-host interface, which can be queried through a simplified and customizable web portal (http://www.metascape.org/IAV). Follow-up studies revealed that the putative ubiquitin ligase UBR4 associates with the viral M2 protein and promotes apical transport of viral proteins. Taken together, the integrative analysis of influenza OMICs datasets illuminates a viral-host network of high-confidence human proteins that are essential for influenza A virus replication.
Copyright ? 2015 Elsevier Inc. All rights reserved.


PMID: 26651948 [PubMed - in process]

 

[tetano]

Cell Host Microbe. 2015 Dec 9;18(6):643-5. doi: 10.1016/j.chom.2015.11.010.
[h=1]Gambling with Flu: "All in" to Maximize Reward.[/h] Stukalov A[SUP]1[/SUP], Pichlmair A[SUP]2[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] In this issue of Cell Host & Microbe, Tripathi et al. (2015) report an in-depth meta-analysis of eight influenza virus siRNA screens combined with viral-host protein interactome data. The integration of the different omics datasets highlights candidate genes and pathways for further investigation and potential therapeutic targeting in the future.
Copyright ? 2015 Elsevier Inc. All rights reserved.


PMID: 26651939 [PubMed - in process]