tetano
Editor, Senior Moderator
J Cell Physiol. 2019 Nov 11. doi: 10.1002/jcp.29392. [Epub ahead of print] [h=1]Metabolic machinery encrypted in the Raman spectrum of influenza A virus-inoculated mammalian cells.[/h]
Pezzotti G[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Zhu W[SUP]1[/SUP], Adachi T[SUP]4,[/SUP][SUP]5[/SUP], Horiguchi S[SUP]4,[/SUP][SUP]5[/SUP], Marin E[SUP]1,[/SUP][SUP]5[/SUP], Boschetto F[SUP]1,[/SUP][SUP]4[/SUP], Ogitani E[SUP]4[/SUP], Mazda O[SUP]4[/SUP].
[h=3]Author information[/h] 1 Faculty of Materials Science and Engineering, Ceramic Physics Laboratory, Kyoto Institute of Technology, Kyoto, Japan. 2 Department of Orthopedic Surgery, Tokyo Medical University, Tokyo, Japan. 3 The Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, Japan. 4 Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. 5 Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
[h=3]Abstract[/h] Raman spectroscopy was applied with a high spectral resolution to a structural study of Influenza (type A) virus before and after its inoculation into Madin-Darby canine kidney cells. This study exploits the fact that the major virus and cell constituents, namely DNA/RNA, lipid, and protein molecules, exhibit peculiar fingerprints in the Raman spectrum, which clearly differed between cells and viruses, as well as before and after virus inoculation into cells. These vibrational features, which allowed us to discuss viral assembly, membrane lipid evolution, and nucleoprotein interactions of the virus with the host cells, reflected the ability of the virus to alter host cells' pathways to enhance its replication efficiency. Upon comparing Raman signals from the host cells before and after virus inoculation, we were also able to discuss in detail cell metabolic reactions against the presence of the virus in terms of compositional variations of lipid species, the formation of fatty acids, dephosphorylation of high-energy adenosine triphosphate molecules, and enzymatic hydrolysis of the hemagglutinin glycoprotein.
? 2019 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] Influenza virus; MDCK cells; Raman spectroscopy; inoculation; metabolic reactions; vibrational fingerprints
PMID: 31710091 DOI: 10.1002/jcp.29392
Pezzotti G[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Zhu W[SUP]1[/SUP], Adachi T[SUP]4,[/SUP][SUP]5[/SUP], Horiguchi S[SUP]4,[/SUP][SUP]5[/SUP], Marin E[SUP]1,[/SUP][SUP]5[/SUP], Boschetto F[SUP]1,[/SUP][SUP]4[/SUP], Ogitani E[SUP]4[/SUP], Mazda O[SUP]4[/SUP].
[h=3]Author information[/h] 1 Faculty of Materials Science and Engineering, Ceramic Physics Laboratory, Kyoto Institute of Technology, Kyoto, Japan. 2 Department of Orthopedic Surgery, Tokyo Medical University, Tokyo, Japan. 3 The Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, Japan. 4 Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. 5 Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
[h=3]Abstract[/h] Raman spectroscopy was applied with a high spectral resolution to a structural study of Influenza (type A) virus before and after its inoculation into Madin-Darby canine kidney cells. This study exploits the fact that the major virus and cell constituents, namely DNA/RNA, lipid, and protein molecules, exhibit peculiar fingerprints in the Raman spectrum, which clearly differed between cells and viruses, as well as before and after virus inoculation into cells. These vibrational features, which allowed us to discuss viral assembly, membrane lipid evolution, and nucleoprotein interactions of the virus with the host cells, reflected the ability of the virus to alter host cells' pathways to enhance its replication efficiency. Upon comparing Raman signals from the host cells before and after virus inoculation, we were also able to discuss in detail cell metabolic reactions against the presence of the virus in terms of compositional variations of lipid species, the formation of fatty acids, dephosphorylation of high-energy adenosine triphosphate molecules, and enzymatic hydrolysis of the hemagglutinin glycoprotein.
? 2019 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] Influenza virus; MDCK cells; Raman spectroscopy; inoculation; metabolic reactions; vibrational fingerprints
PMID: 31710091 DOI: 10.1002/jcp.29392