tetano
Editor, Senior Moderator
Biosens Bioelectron. 2014 Feb 22;58C:33-39. doi: 10.1016/j.bios.2014.02.039. [Epub ahead of print]
Metal enhanced fluorescence on nanoporous gold leaf-based assay platform for virus detection.
Ahmed SR1, Hossain MA2, Park JY3, Kim SH4, Lee D5, Suzuki T6, Lee J7, Park EY8.
Author information
Abstract
In the present study, a rapid, sensitive and quantitative detection of influenza A virus targeting hemagglutinin (HA) was developed using hybrid structure of quantum dots (QDs) and nanoporous gold leaf (NPGL). NPGL film was prepared by dealloying bimetallic film where its surface morphology and roughness were fairly controlled. Anti-influenza A virus HA antibody (ab66189) was bound with NPGL and amine (-NH2) terminated QDs. These biofunctionalized NPGL and QDs formed a complex with the influenza virus A/Beijing/262/95 (H1N1) and the photoluminescence (PL) intensities of QDs were linearly correlated with the concentrations of the virus up to 1ng/mL while no PL was observed in the absence of the virus, or in bovine serum albumin (BSA, 1?g/mL) alone. In addition, it was demonstrated that this assay detected successfully influenza virus A/Yokohama/110/2009 (H3N2) that is isolated from a clinical sample, at a concentration of ca. 50 plaque forming units (PFU)/mL. This detection limit is 2-order more sensitive than a commercially available rapid influenza diagnostic test. From these results, the proposed assay may offer a new strategy to monitor influenza virus for public health.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Fluorescence enhancement, Influenza A virus, Nanoporous gold leaf, Quantum dot, Surface roughness
PMID:
24607620
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24607620
Metal enhanced fluorescence on nanoporous gold leaf-based assay platform for virus detection.
Ahmed SR1, Hossain MA2, Park JY3, Kim SH4, Lee D5, Suzuki T6, Lee J7, Park EY8.
Author information
Abstract
In the present study, a rapid, sensitive and quantitative detection of influenza A virus targeting hemagglutinin (HA) was developed using hybrid structure of quantum dots (QDs) and nanoporous gold leaf (NPGL). NPGL film was prepared by dealloying bimetallic film where its surface morphology and roughness were fairly controlled. Anti-influenza A virus HA antibody (ab66189) was bound with NPGL and amine (-NH2) terminated QDs. These biofunctionalized NPGL and QDs formed a complex with the influenza virus A/Beijing/262/95 (H1N1) and the photoluminescence (PL) intensities of QDs were linearly correlated with the concentrations of the virus up to 1ng/mL while no PL was observed in the absence of the virus, or in bovine serum albumin (BSA, 1?g/mL) alone. In addition, it was demonstrated that this assay detected successfully influenza virus A/Yokohama/110/2009 (H3N2) that is isolated from a clinical sample, at a concentration of ca. 50 plaque forming units (PFU)/mL. This detection limit is 2-order more sensitive than a commercially available rapid influenza diagnostic test. From these results, the proposed assay may offer a new strategy to monitor influenza virus for public health.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Fluorescence enhancement, Influenza A virus, Nanoporous gold leaf, Quantum dot, Surface roughness
PMID:
24607620
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24607620