tetano
Editor, Senior Moderator
Indian J Microbiol. 2014 Jun;54(2):211-7. doi: 10.1007/s12088-013-0432-x. Epub 2013 Oct 22.
Microarray multiplex assay for the simultaneous detection and discrimination of influenza a and influenza B viruses.
Tian M1, Tian Y1, Li Y2, Lu H3, Li X3, Li C3, Xue F3, Jin N3.
Author information
Abstract
In this study, we present a microarray approach for the typing of influenza A and B viruses, and the subtyping of H1 and H3 subtypes. We designed four pairs of specific multiplex RT-PCR primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. Through amplification and fluorescent marking of the multiplex RT-PCR products on the M gene of influenza A and B viruses and the HA gene of subtypes H1 and H3, the PCR products were hybridized with the microarray, and the results were analyzed using a microarray scanner. The results demonstrate that the chip developed by our research institute can detect influenza A and B viruses specifically and identify the subtypes H1 and H3 at a minimum concentration of 1 ? 10(2) copies/μL of viral RNA. We tested 35 clinical samples and our results were identical to other fluorescent methods. The microarray approach developed in this study provides a reliable method for the monitoring and testing of seasonal influenza.
KEYWORDS:
DNA microarray; Influenza A virus; Influenza B virus; Typing
PMID:
25320424
[PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/25320424
Microarray multiplex assay for the simultaneous detection and discrimination of influenza a and influenza B viruses.
Tian M1, Tian Y1, Li Y2, Lu H3, Li X3, Li C3, Xue F3, Jin N3.
Author information
Abstract
In this study, we present a microarray approach for the typing of influenza A and B viruses, and the subtyping of H1 and H3 subtypes. We designed four pairs of specific multiplex RT-PCR primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. Through amplification and fluorescent marking of the multiplex RT-PCR products on the M gene of influenza A and B viruses and the HA gene of subtypes H1 and H3, the PCR products were hybridized with the microarray, and the results were analyzed using a microarray scanner. The results demonstrate that the chip developed by our research institute can detect influenza A and B viruses specifically and identify the subtypes H1 and H3 at a minimum concentration of 1 ? 10(2) copies/μL of viral RNA. We tested 35 clinical samples and our results were identical to other fluorescent methods. The microarray approach developed in this study provides a reliable method for the monitoring and testing of seasonal influenza.
KEYWORDS:
DNA microarray; Influenza A virus; Influenza B virus; Typing
PMID:
25320424
[PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/25320424