tetano
Editor, Senior Moderator
Microbes Infect
. 2022 Feb 2;104949.
doi: 10.1016/j.micinf.2022.104949. Online ahead of print.
Clinical and genomic data of sars-cov-2 detected in maternal-fetal interface during the first wave of infection in brazil
Maria de Fátima Carvalho Ferreira[SUP] 1 [/SUP], Janeth Aracely Ramirez Pavon[SUP] 2 [/SUP], Amanda Colichio Bini Napoleão[SUP] 1 [/SUP], Gláucia Maria Duarte Preza Figueiredo[SUP] 1 [/SUP], Patricia Cristina Borges Florêncio[SUP] 1 [/SUP], Rayssa Basílio Dos Santos Arantes[SUP] 1 [/SUP], Paula Sossai Rizzo[SUP] 1 [/SUP], Maria Aparecida Mazzutti Verlangieri Carmo[SUP] 1 [/SUP], Luciano Nakazato[SUP] 3 [/SUP], Valéria Dutra[SUP] 3 [/SUP], Rosane Christine Hahn[SUP] 4 [/SUP], Renata Dezengrini Slhessarenko[SUP] 5 [/SUP]
Affiliations
Abstract
Brazil has the highest SARS-CoV-2 case-fatality rate in pregnant women in the Americas. In this study, clinical and virological findings of five mildly symptomatic pregnant women and their infected fetuses/newborns treated at a referral hospital for COVID19-pregnant women in Midwestern Brazil are reported. Mother and fetal samples were tested by RT-qPCR, ECLIA and Illumina MiSeq sequencing. From the five cases, one resulted in spontaneous abortion, one was stillborn, two were preterm births and one full-term birth. Maternal and fetal placenta, newborn and stillborn secretions were SARS-CoV-2+; one neonate developed ground-glass opacities in his lungs. One neonate's umbilical cord was IgG+ and all were IgM negative upon hospital discharge. Genomes recovered from two placentas belong to the B.1.1.28 and B.1.1.33 lineages and present nonsynonymous mutations associated with virus fitness and infectivity; other not frequently reported mutations (B.1.1.33: NSP3 V2090G, M A2S and ORF3ab S253P and Y264N; B.1.1.28: NSP3 E995D, NSP12 R240K, NSP14 H1897Y and in ORF7b V21F) were found in proteins involved in viral replication, viral induction of apoptosis, viral interference on interferon and on NF-Κβ pathways. Phylogeny indicates the south of Brazil as the possible origin of these lineages circulating in MT. These findings contribute to describe SARS-CoV-2 infection and outcomes in pregnant women and their fetuses, at any stage of gestation and even in mild symptomatic cases.
Keywords: clinical virology; coronavirus; molecular virology; neonatal infection; vertical transmission.
. 2022 Feb 2;104949.
doi: 10.1016/j.micinf.2022.104949. Online ahead of print.
Clinical and genomic data of sars-cov-2 detected in maternal-fetal interface during the first wave of infection in brazil
Maria de Fátima Carvalho Ferreira[SUP] 1 [/SUP], Janeth Aracely Ramirez Pavon[SUP] 2 [/SUP], Amanda Colichio Bini Napoleão[SUP] 1 [/SUP], Gláucia Maria Duarte Preza Figueiredo[SUP] 1 [/SUP], Patricia Cristina Borges Florêncio[SUP] 1 [/SUP], Rayssa Basílio Dos Santos Arantes[SUP] 1 [/SUP], Paula Sossai Rizzo[SUP] 1 [/SUP], Maria Aparecida Mazzutti Verlangieri Carmo[SUP] 1 [/SUP], Luciano Nakazato[SUP] 3 [/SUP], Valéria Dutra[SUP] 3 [/SUP], Rosane Christine Hahn[SUP] 4 [/SUP], Renata Dezengrini Slhessarenko[SUP] 5 [/SUP]
Affiliations
- PMID: 35123044
- DOI: 10.1016/j.micinf.2022.104949
Abstract
Brazil has the highest SARS-CoV-2 case-fatality rate in pregnant women in the Americas. In this study, clinical and virological findings of five mildly symptomatic pregnant women and their infected fetuses/newborns treated at a referral hospital for COVID19-pregnant women in Midwestern Brazil are reported. Mother and fetal samples were tested by RT-qPCR, ECLIA and Illumina MiSeq sequencing. From the five cases, one resulted in spontaneous abortion, one was stillborn, two were preterm births and one full-term birth. Maternal and fetal placenta, newborn and stillborn secretions were SARS-CoV-2+; one neonate developed ground-glass opacities in his lungs. One neonate's umbilical cord was IgG+ and all were IgM negative upon hospital discharge. Genomes recovered from two placentas belong to the B.1.1.28 and B.1.1.33 lineages and present nonsynonymous mutations associated with virus fitness and infectivity; other not frequently reported mutations (B.1.1.33: NSP3 V2090G, M A2S and ORF3ab S253P and Y264N; B.1.1.28: NSP3 E995D, NSP12 R240K, NSP14 H1897Y and in ORF7b V21F) were found in proteins involved in viral replication, viral induction of apoptosis, viral interference on interferon and on NF-Κβ pathways. Phylogeny indicates the south of Brazil as the possible origin of these lineages circulating in MT. These findings contribute to describe SARS-CoV-2 infection and outcomes in pregnant women and their fetuses, at any stage of gestation and even in mild symptomatic cases.
Keywords: clinical virology; coronavirus; molecular virology; neonatal infection; vertical transmission.