tetano
Editor, Senior Moderator
Microbiol Spectr
. 2024 Jan 8:e0353023.
doi: 10.1128/spectrum.03530-23. Online ahead of print. Genome-wide bioinformatics analysis of human protease capacity for proteolytic cleavage of the SARS-CoV-2 spike glycoprotein
Evgenii V Matveev[SUP] 1 2 3 [/SUP], Gennady V Ponomarev[SUP] 1 2 [/SUP], Marat D Kazanov[SUP] 1 2 3 4 [/SUP]
Affiliations
The entry of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) into the cell, activated by host proteases, is considerably more complex in coronaviruses than in most other viruses and is not fully understood. There is evidence that other proteases beyond the known furin and transmembrane protease serine 2 can activate the spike protein. Another example of uncertainty is the cleavage site for the alternative endocytic route of SARS-CoV-2 entrance, which is still unknown. Bioinformatics methods, modeling protease specificity and estimating the structural susceptibility of protein regions to proteolysis, can aid in studying this topic by predicting the involved proteases and their cleavage sites, thereby substantially reducing the amount of experimental work. Elucidating the mechanisms of spike protein activation is crucial for preventing possible future coronavirus pandemics and developing antiviral drugs.
Keywords: PCSK; SARS-CoV-2; TMPRSS2; TTSP; cathepsin L; furin; protease; proteolysis; proteolytic activation; spike glycoprotein.
. 2024 Jan 8:e0353023.
doi: 10.1128/spectrum.03530-23. Online ahead of print. Genome-wide bioinformatics analysis of human protease capacity for proteolytic cleavage of the SARS-CoV-2 spike glycoprotein
Evgenii V Matveev[SUP] 1 2 3 [/SUP], Gennady V Ponomarev[SUP] 1 2 [/SUP], Marat D Kazanov[SUP] 1 2 3 4 [/SUP]
Affiliations
- PMID: 38189333
- DOI: 10.1128/spectrum.03530-23
The entry of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) into the cell, activated by host proteases, is considerably more complex in coronaviruses than in most other viruses and is not fully understood. There is evidence that other proteases beyond the known furin and transmembrane protease serine 2 can activate the spike protein. Another example of uncertainty is the cleavage site for the alternative endocytic route of SARS-CoV-2 entrance, which is still unknown. Bioinformatics methods, modeling protease specificity and estimating the structural susceptibility of protein regions to proteolysis, can aid in studying this topic by predicting the involved proteases and their cleavage sites, thereby substantially reducing the amount of experimental work. Elucidating the mechanisms of spike protein activation is crucial for preventing possible future coronavirus pandemics and developing antiviral drugs.
Keywords: PCSK; SARS-CoV-2; TMPRSS2; TTSP; cathepsin L; furin; protease; proteolysis; proteolytic activation; spike glycoprotein.