tetano
Editor, Senior Moderator
Microbiol Spectr
. 2023 Dec 12:e0298223.
doi: 10.1128/spectrum.02982-23. Online ahead of print. Targeted amplification and genetic sequencing of the severe acute respiratory syndrome coronavirus 2 surface glycoprotein
Matthew W Keller[SUP] 1 [/SUP], Lisa M Keong[SUP] 1 [/SUP], Benjamin L Rambo-Martin[SUP] 1 [/SUP], Norman Hassell[SUP] 1 [/SUP], Kristine A Lacek[SUP] 1 [/SUP], Malania M Wilson[SUP] 1 [/SUP], Marie K Kirby[SUP] 1 [/SUP], Jimma Liddell[SUP] 1 [/SUP], D Collins Owuor[SUP] 1 [/SUP], Mili Sheth[SUP] 2 [/SUP], Joseph Madden[SUP] 2 [/SUP], Justin S Lee[SUP] 2 [/SUP], Rebecca J Kondor[SUP] 1 [/SUP], David E Wentworth[SUP] 1 [/SUP], John R Barnes[SUP] 1 [/SUP]
Affiliations
The COVID-19 pandemic was accompanied by an unprecedented surveillance effort. The resulting data were and will continue to be critical for surveillance and control of SARS-CoV-2. However, some genomic surveillance methods experienced challenges as the virus evolved, resulting in incomplete and poor quality data. Complete and quality coverage, especially of the S-gene, is important for supporting the selection of vaccine candidates. As such, we developed a robust method to target the S-gene for amplification and sequencing. By focusing on the S-gene and imposing strict coverage and quality metrics, we hope to increase the quality of surveillance data for this continually evolving gene. Our technique is currently being deployed globally to partner laboratories, and public health representatives from 79 countries have received hands-on training and support. Expanding access to quality surveillance methods will undoubtedly lead to earlier detection of novel variants and better inform vaccine strain selection.
Keywords: SARS-CoV-2; gene sequencing; surveillance studies.
. 2023 Dec 12:e0298223.
doi: 10.1128/spectrum.02982-23. Online ahead of print. Targeted amplification and genetic sequencing of the severe acute respiratory syndrome coronavirus 2 surface glycoprotein
Matthew W Keller[SUP] 1 [/SUP], Lisa M Keong[SUP] 1 [/SUP], Benjamin L Rambo-Martin[SUP] 1 [/SUP], Norman Hassell[SUP] 1 [/SUP], Kristine A Lacek[SUP] 1 [/SUP], Malania M Wilson[SUP] 1 [/SUP], Marie K Kirby[SUP] 1 [/SUP], Jimma Liddell[SUP] 1 [/SUP], D Collins Owuor[SUP] 1 [/SUP], Mili Sheth[SUP] 2 [/SUP], Joseph Madden[SUP] 2 [/SUP], Justin S Lee[SUP] 2 [/SUP], Rebecca J Kondor[SUP] 1 [/SUP], David E Wentworth[SUP] 1 [/SUP], John R Barnes[SUP] 1 [/SUP]
Affiliations
- PMID: 38084972
- DOI: 10.1128/spectrum.02982-23
The COVID-19 pandemic was accompanied by an unprecedented surveillance effort. The resulting data were and will continue to be critical for surveillance and control of SARS-CoV-2. However, some genomic surveillance methods experienced challenges as the virus evolved, resulting in incomplete and poor quality data. Complete and quality coverage, especially of the S-gene, is important for supporting the selection of vaccine candidates. As such, we developed a robust method to target the S-gene for amplification and sequencing. By focusing on the S-gene and imposing strict coverage and quality metrics, we hope to increase the quality of surveillance data for this continually evolving gene. Our technique is currently being deployed globally to partner laboratories, and public health representatives from 79 countries have received hands-on training and support. Expanding access to quality surveillance methods will undoubtedly lead to earlier detection of novel variants and better inform vaccine strain selection.
Keywords: SARS-CoV-2; gene sequencing; surveillance studies.