tetano
Editor, Senior Moderator
PLoS One. 2015 Jun 24;10(6):e0131531. doi: 10.1371/journal.pone.0131531. eCollection 2015.
[h=1]Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children.[/h] Verschoor CP[SUP]1[/SUP], Singh P[SUP]2[/SUP], Russell ML[SUP]3[/SUP], Bowdish DM[SUP]1[/SUP], Brewer A[SUP]4[/SUP], Cyr L[SUP]4[/SUP], Ward BJ[SUP]4[/SUP], Loeb M[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by MN and hemagglutination inhibition (HAI) assays. Compared to HAI, the MN assay is more sensitive in detecting serum antibodies and estimates of protective effectiveness against PCR-confirmed infection were higher for both subtypes. Given our findings, the MN assay warrants further consideration as a formal tool for the routine evaluation of vaccine-induced antibody responses.
PMID: 26107625 [PubMed - in process] Free full text
[h=1]Microneutralization Assay Titres Correlate with Protection against Seasonal Influenza H1N1 and H3N2 in Children.[/h] Verschoor CP[SUP]1[/SUP], Singh P[SUP]2[/SUP], Russell ML[SUP]3[/SUP], Bowdish DM[SUP]1[/SUP], Brewer A[SUP]4[/SUP], Cyr L[SUP]4[/SUP], Ward BJ[SUP]4[/SUP], Loeb M[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although the microneutralization (MN) assay has been shown to be more sensitive than the hemagglutination inhibition (HAI) assay for the measurement of humoral immunity against influenza viruses, further evidence relating MN titres to protective efficacy against infection is needed. Serum antibodies against seasonal H1N1 and H3N2 influenza were measured in children and adolescents (n = 656) by MN and hemagglutination inhibition (HAI) assays. Compared to HAI, the MN assay is more sensitive in detecting serum antibodies and estimates of protective effectiveness against PCR-confirmed infection were higher for both subtypes. Given our findings, the MN assay warrants further consideration as a formal tool for the routine evaluation of vaccine-induced antibody responses.
PMID: 26107625 [PubMed - in process] Free full text