tetano
Editor, Senior Moderator
Microorganisms
. 2021 Jun 10;9(6):1263.
doi: 10.3390/microorganisms9061263.
Combination of rRT-PCR and Anti-Nucleocapsid/Anti-Spike Antibodies to Characterize Specimens with Very Low Viral SARs-CoV-2 Load: A Real-Life Experience
Zoe Florou[SUP] 1 [/SUP], Meropi Zigra[SUP] 1 [/SUP], Philippos Kartalidis[SUP] 1 [/SUP], Katerina Tsilipounidaki[SUP] 1 [/SUP], Georgia Papadamou[SUP] 2 [/SUP], Aikaterini Belia[SUP] 3 [/SUP], George C Fthenakis[SUP] 4 [/SUP], Efthymia Petinaki[SUP] 1 [/SUP]
Affiliations
Abstract
The objective of the present study was to evaluate the true positivity among people, whose results of initial testing of nasopharyngeal swabs (NPS) showed a very low viral load of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Seventy-seven people detected with low viral loads of SARs-CoV-2 in nasopharyngeal samples (Ct ≥ 35) were enrolled in the study. For this purpose, a second NPS was collected for rRT-PCR (real-time reverse transcription polymerase chain reaction) combined with a pair of serum samples for detection of anti-nucleocapsid (anti-N) and anti-spike (anti-S) antibodies. In 8 people, subsequent examinations indicated an increase in viral loads, thereafter, followed by an increase of anti-N and anti-S antibodies, findings compatible with an early stage of COVID-19 infection. In 9 people, who already had increased anti-N antibodies, subsequent examination showed a decrease or absence of viral load and an increase in antibodies, indicative of a late stage of COVID-19 infection. In 60 people, subsequent examination showed absence of infection (as indicated by absence of viral load and antibodies). We propose that the combination of a second NPS and one serum-specimen, both taken three days after the first NPS, helps significantly to avoid false-positive results.
Keywords: COVID-19; Greece; N gene; anti-nucleocapsid antibodies; anti-spike antibodies; diagnosis; false positive; low viral load.
. 2021 Jun 10;9(6):1263.
doi: 10.3390/microorganisms9061263.
Combination of rRT-PCR and Anti-Nucleocapsid/Anti-Spike Antibodies to Characterize Specimens with Very Low Viral SARs-CoV-2 Load: A Real-Life Experience
Zoe Florou[SUP] 1 [/SUP], Meropi Zigra[SUP] 1 [/SUP], Philippos Kartalidis[SUP] 1 [/SUP], Katerina Tsilipounidaki[SUP] 1 [/SUP], Georgia Papadamou[SUP] 2 [/SUP], Aikaterini Belia[SUP] 3 [/SUP], George C Fthenakis[SUP] 4 [/SUP], Efthymia Petinaki[SUP] 1 [/SUP]
Affiliations
- PMID: 34200837
- DOI: 10.3390/microorganisms9061263
Abstract
The objective of the present study was to evaluate the true positivity among people, whose results of initial testing of nasopharyngeal swabs (NPS) showed a very low viral load of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Seventy-seven people detected with low viral loads of SARs-CoV-2 in nasopharyngeal samples (Ct ≥ 35) were enrolled in the study. For this purpose, a second NPS was collected for rRT-PCR (real-time reverse transcription polymerase chain reaction) combined with a pair of serum samples for detection of anti-nucleocapsid (anti-N) and anti-spike (anti-S) antibodies. In 8 people, subsequent examinations indicated an increase in viral loads, thereafter, followed by an increase of anti-N and anti-S antibodies, findings compatible with an early stage of COVID-19 infection. In 9 people, who already had increased anti-N antibodies, subsequent examination showed a decrease or absence of viral load and an increase in antibodies, indicative of a late stage of COVID-19 infection. In 60 people, subsequent examination showed absence of infection (as indicated by absence of viral load and antibodies). We propose that the combination of a second NPS and one serum-specimen, both taken three days after the first NPS, helps significantly to avoid false-positive results.
Keywords: COVID-19; Greece; N gene; anti-nucleocapsid antibodies; anti-spike antibodies; diagnosis; false positive; low viral load.