tetano
Editor, Senior Moderator
Microorganisms
. 2021 Mar 31;9(4):733.
doi: 10.3390/microorganisms9040733.
Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG
Kirsten Alexandra Eberhardt[SUP] 1 2 [/SUP], Felix Dewald[SUP] 3 4 [/SUP], Eva Heger[SUP] 3 [/SUP], Lutz Gieselmann[SUP] 3 [/SUP], Kanika Vanshylla[SUP] 3 4 [/SUP], Maike Wirtz[SUP] 3 [/SUP], Franziska Kleipass[SUP] 3 [/SUP], Wibke Johannis[SUP] 5 [/SUP], Philipp Schommers[SUP] 3 6 7 [/SUP], Henning Gruell[SUP] 3 7 [/SUP], Karl August Brensing[SUP] 8 [/SUP], Roman-Ulrich M?ller[SUP] 4 9 10 [/SUP], Max Augustin[SUP] 6 [/SUP], Clara Lehmann[SUP] 4 6 7 [/SUP], Manuel Koch[SUP] 4 11 [/SUP], Florian Klein[SUP] 3 4 7 [/SUP], Veronica Di Cristanziano[SUP] 3 4 [/SUP]
Affiliations
Abstract
Background: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK? anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK[SUP]?[/SUP] anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON[SUP]?[/SUP] SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys[SUP]?[/SUP] anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK? anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK[SUP]?[/SUP] anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.
Keywords: COVID-19; antibody; coronavirus; humoral response; neutralization; non-responder; pandemic; protection; sensitivity; titer.
. 2021 Mar 31;9(4):733.
doi: 10.3390/microorganisms9040733.
Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG
Kirsten Alexandra Eberhardt[SUP] 1 2 [/SUP], Felix Dewald[SUP] 3 4 [/SUP], Eva Heger[SUP] 3 [/SUP], Lutz Gieselmann[SUP] 3 [/SUP], Kanika Vanshylla[SUP] 3 4 [/SUP], Maike Wirtz[SUP] 3 [/SUP], Franziska Kleipass[SUP] 3 [/SUP], Wibke Johannis[SUP] 5 [/SUP], Philipp Schommers[SUP] 3 6 7 [/SUP], Henning Gruell[SUP] 3 7 [/SUP], Karl August Brensing[SUP] 8 [/SUP], Roman-Ulrich M?ller[SUP] 4 9 10 [/SUP], Max Augustin[SUP] 6 [/SUP], Clara Lehmann[SUP] 4 6 7 [/SUP], Manuel Koch[SUP] 4 11 [/SUP], Florian Klein[SUP] 3 4 7 [/SUP], Veronica Di Cristanziano[SUP] 3 4 [/SUP]
Affiliations
- PMID: 33807490
- DOI: 10.3390/microorganisms9040733
Abstract
Background: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK? anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK[SUP]?[/SUP] anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON[SUP]?[/SUP] SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys[SUP]?[/SUP] anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK? anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK[SUP]?[/SUP] anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.
Keywords: COVID-19; antibody; coronavirus; humoral response; neutralization; non-responder; pandemic; protection; sensitivity; titer.