tetano
Editor, Senior Moderator
PLoS One. 2017 Aug 23;12(8):e0183717. doi: 10.1371/journal.pone.0183717. eCollection 2017.
[h=1]Microparticle and anti-influenza activity in human respiratory secretion.[/h] Suptawiwat O[SUP]1[/SUP], Ruangrung K[SUP]1[/SUP], Boonarkart C[SUP]1[/SUP], Puthavathana P[SUP]2[/SUP], Maneechotesuwan K[SUP]3[/SUP], Charngkaew K[SUP]4[/SUP], Chomanee N[SUP]4[/SUP], Auewarakul P[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert anti-influenza activity and are believed to be responsible for the anti-influenza activity in respiratory secretions. It was previously shown that a bronchial epithelial cell culture could produce exosome-like particles with anti-influenza activity. Whether such extracellular vesicles in respiratory secretions have anti-influenza activity is unknown. Therefore, we characterized bronchoalveolar lavage fluid and found microparticles, which mostly stained positive for epithelial cell markers and both α2,3- and α2,6-linked sialic acid. Microparticles were purified from bronchoalveolar lavage fluid and shown to exhibit anti-influenza activity by a hemagglutination inhibition (HI) assay and a neutralization (NT) assay. In addition, physical binding between influenza virions and microparticles was demonstrated by electron microscopy. These findings indicate that respiratory microparticles containing viral receptors can exert anti-viral activity by probably trapping viral particles. This innate mechanism may play an important role in the defense against respiratory viruses.
PMID: 28832645 DOI: 10.1371/journal.pone.0183717
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[h=1]Microparticle and anti-influenza activity in human respiratory secretion.[/h] Suptawiwat O[SUP]1[/SUP], Ruangrung K[SUP]1[/SUP], Boonarkart C[SUP]1[/SUP], Puthavathana P[SUP]2[/SUP], Maneechotesuwan K[SUP]3[/SUP], Charngkaew K[SUP]4[/SUP], Chomanee N[SUP]4[/SUP], Auewarakul P[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert anti-influenza activity and are believed to be responsible for the anti-influenza activity in respiratory secretions. It was previously shown that a bronchial epithelial cell culture could produce exosome-like particles with anti-influenza activity. Whether such extracellular vesicles in respiratory secretions have anti-influenza activity is unknown. Therefore, we characterized bronchoalveolar lavage fluid and found microparticles, which mostly stained positive for epithelial cell markers and both α2,3- and α2,6-linked sialic acid. Microparticles were purified from bronchoalveolar lavage fluid and shown to exhibit anti-influenza activity by a hemagglutination inhibition (HI) assay and a neutralization (NT) assay. In addition, physical binding between influenza virions and microparticles was demonstrated by electron microscopy. These findings indicate that respiratory microparticles containing viral receptors can exert anti-viral activity by probably trapping viral particles. This innate mechanism may play an important role in the defense against respiratory viruses.
PMID: 28832645 DOI: 10.1371/journal.pone.0183717
Free full text