tetano
Editor, Senior Moderator
Mol Biol Rep
. 2020 Oct 6.
doi: 10.1007/s11033-020-05889-3. Online ahead of print.
Recent advances in the nucleic acid-based diagnostic tool for coronavirus
Pranav Kumar Prabhakar[SUP] 1 [/SUP], Jyoti Lakhanpal[SUP] 2 [/SUP]
Affiliations
Abstract
Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
Keywords: Amplification; COVID-19; Coronavirus; Microarray; Nucleic acid detection; qRT-PCR.
. 2020 Oct 6.
doi: 10.1007/s11033-020-05889-3. Online ahead of print.
Recent advances in the nucleic acid-based diagnostic tool for coronavirus
Pranav Kumar Prabhakar[SUP] 1 [/SUP], Jyoti Lakhanpal[SUP] 2 [/SUP]
Affiliations
- PMID: 33025503
- DOI: 10.1007/s11033-020-05889-3
Abstract
Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
Keywords: Amplification; COVID-19; Coronavirus; Microarray; Nucleic acid detection; qRT-PCR.