tetano
Editor, Senior Moderator
Mol Pharm
. 2023 Feb 16.
doi: 10.1021/acs.molpharmaceut.2c00805. Online ahead of print.
Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens
Yue Zhang[SUP] 1 2 [/SUP], Tianyu Zhu[SUP] 1 2 [/SUP], Shuwen Xu[SUP] 1 2 [/SUP], Pengfei Gu[SUP] 1 2 [/SUP], Gaofeng Cai[SUP] 1 2 [/SUP], Song Peng[SUP] 1 2 [/SUP], Zhenguang Liu[SUP] 1 2 [/SUP], Yang Yang[SUP] 1 2 [/SUP], Yuanliang Hu[SUP] 1 2 [/SUP], Jiaguo Liu[SUP] 1 2 [/SUP], Deyun Wang[SUP] 1 2 [/SUP]
Affiliations
Abstract
Chinese yam polysaccharides (CYPs) have received wide attention for their immunomodulatory activity. Our previous studies had discovered that the Chinese yam polysaccharide PLGA-stabilized Pickering emulsion (CYP-PPAS) can serve as an efficient adjuvant to trigger powerful humoral and cellular immunity. Recently, positively charged nano-adjuvants are easily taken up by antigen-presenting cells, potentially resulting in lysosomal escape, the promotion of antigen cross-presentation, and the induction of CD8 T-cell response. However, reports on the practical application of cationic Pickering emulsions as adjuvants are very limited. Considering the economic damage and public-health risks caused by the H9N2 influenza virus, it is urgent to develop an effective adjuvant for boosting humoral and cellular immunity against influenza virus infection. Here, we applied polyethyleneimine-modified Chinese yam polysaccharide PLGA nanoparticles as particle stabilizers and squalene as the oil core to fabricate a positively charged nanoparticle-stabilized Pickering emulsion adjuvant system (PEI-CYP-PPAS). The cationic Pickering emulsion of PEI-CYP-PPAS was utilized as an adjuvant for the H9N2 Avian influenza vaccine, and the adjuvant activity was compared with the Pickering emulsion of CYP-PPAS and the commercial adjuvant (aluminum adjuvant). The PEI-CYP-PPAS, with a size of about 1164.66 nm and a ζ potential of 33.23 mV, could increase the H9N2 antigen loading efficiency by 83.99%. After vaccination with Pickering emulsions based on H9N2 vaccines, PEI-CYP-PPAS generated higher HI titers and stronger IgG antibodies than CYP-PPAS and Alum and increased the immune organ index of the spleen and bursa of Fabricius without immune organ injury. Moreover, treatment with PEI-CYP-PPAS/H9N2 induced CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell activation, a high lymphocyte proliferation index, and increased cytokine expression of IL-4, IL-6, and IFN-γ. Thus, compared with the CYP-PPAS and aluminum adjuvant, the cationic nanoparticle-stabilized vaccine delivery system of PEI-CYP-PPAS was an effective adjuvant for H9N2 vaccination to elicit powerful humoral and cellular immune responses.
Keywords: Chinese yam polysaccharides; Pickering emulsion; adjuvant; avian influenza viruses; poly (lactic-co-glycolic acid); polyethyleneimine.
. 2023 Feb 16.
doi: 10.1021/acs.molpharmaceut.2c00805. Online ahead of print.
Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens
Yue Zhang[SUP] 1 2 [/SUP], Tianyu Zhu[SUP] 1 2 [/SUP], Shuwen Xu[SUP] 1 2 [/SUP], Pengfei Gu[SUP] 1 2 [/SUP], Gaofeng Cai[SUP] 1 2 [/SUP], Song Peng[SUP] 1 2 [/SUP], Zhenguang Liu[SUP] 1 2 [/SUP], Yang Yang[SUP] 1 2 [/SUP], Yuanliang Hu[SUP] 1 2 [/SUP], Jiaguo Liu[SUP] 1 2 [/SUP], Deyun Wang[SUP] 1 2 [/SUP]
Affiliations
- PMID: 36795759
- DOI: 10.1021/acs.molpharmaceut.2c00805
Abstract
Chinese yam polysaccharides (CYPs) have received wide attention for their immunomodulatory activity. Our previous studies had discovered that the Chinese yam polysaccharide PLGA-stabilized Pickering emulsion (CYP-PPAS) can serve as an efficient adjuvant to trigger powerful humoral and cellular immunity. Recently, positively charged nano-adjuvants are easily taken up by antigen-presenting cells, potentially resulting in lysosomal escape, the promotion of antigen cross-presentation, and the induction of CD8 T-cell response. However, reports on the practical application of cationic Pickering emulsions as adjuvants are very limited. Considering the economic damage and public-health risks caused by the H9N2 influenza virus, it is urgent to develop an effective adjuvant for boosting humoral and cellular immunity against influenza virus infection. Here, we applied polyethyleneimine-modified Chinese yam polysaccharide PLGA nanoparticles as particle stabilizers and squalene as the oil core to fabricate a positively charged nanoparticle-stabilized Pickering emulsion adjuvant system (PEI-CYP-PPAS). The cationic Pickering emulsion of PEI-CYP-PPAS was utilized as an adjuvant for the H9N2 Avian influenza vaccine, and the adjuvant activity was compared with the Pickering emulsion of CYP-PPAS and the commercial adjuvant (aluminum adjuvant). The PEI-CYP-PPAS, with a size of about 1164.66 nm and a ζ potential of 33.23 mV, could increase the H9N2 antigen loading efficiency by 83.99%. After vaccination with Pickering emulsions based on H9N2 vaccines, PEI-CYP-PPAS generated higher HI titers and stronger IgG antibodies than CYP-PPAS and Alum and increased the immune organ index of the spleen and bursa of Fabricius without immune organ injury. Moreover, treatment with PEI-CYP-PPAS/H9N2 induced CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell activation, a high lymphocyte proliferation index, and increased cytokine expression of IL-4, IL-6, and IFN-γ. Thus, compared with the CYP-PPAS and aluminum adjuvant, the cationic nanoparticle-stabilized vaccine delivery system of PEI-CYP-PPAS was an effective adjuvant for H9N2 vaccination to elicit powerful humoral and cellular immune responses.
Keywords: Chinese yam polysaccharides; Pickering emulsion; adjuvant; avian influenza viruses; poly (lactic-co-glycolic acid); polyethyleneimine.