tetano
Editor, Senior Moderator
Liposome Res. 2017 Sep 18:1-31. doi: 10.1080/08982104.2017.1381708. [Epub ahead of print] [h=1]Monomeric M2e antigen in VesiVax? liposomes stimulates protection against type A strains of influenza comparable to liposomes with multimeric forms of M2e.[/h] Adler-Moore JP[SUP]1[/SUP], Ernst W[SUP]2[/SUP], Kim H[SUP]1[/SUP], Ward N[SUP]1[/SUP], Chiang SM[SUP]1[/SUP], Do T[SUP]2[/SUP], Fujii G[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Given the interest in the ectodomain of the matrix 2 (M2e) channel protein as a target for development of a universal influenza vaccine, we examined the role of the antigen configuration of M2e in generating a protective immune response. A series of M2e mutations and a truncated M2e segment were prepared as a means of controlling the formation of monomer, dimer, and higher order multimeric forms of M2e. Each of these M2e peptides was incorporated into a liposome-based vaccine technology platform previously shown to stimulate a protective response to influenza A infection using M2e as a mixture of monomers, dimers and multimers (L-M2e1-HD/MPL). Our results using these modified forms of M2e produced 90%-100% survival following lethal challenge with H1N1 (A/PR/8/34) in both inbred BALB/c and outbred Swiss Webster mice vaccinated with a truncated monomeric form of the M2 protein, M2e[SUB]1-15[/SUB] in liposomes. These observations show that a tetrameric configuration is not required to elicit significant protection when the M2e antigen is formulated in immunogenic liposomes and further, that the first 15 amino acids of M2e likely play a primary role in providing the protective immune response.
[h=4]KEYWORDS:[/h] influenza M2e; liposomes; vaccination
PMID: 28922045 DOI: 10.1080/08982104.2017.1381708
[h=3]Author information[/h]
[h=3]Abstract[/h] Given the interest in the ectodomain of the matrix 2 (M2e) channel protein as a target for development of a universal influenza vaccine, we examined the role of the antigen configuration of M2e in generating a protective immune response. A series of M2e mutations and a truncated M2e segment were prepared as a means of controlling the formation of monomer, dimer, and higher order multimeric forms of M2e. Each of these M2e peptides was incorporated into a liposome-based vaccine technology platform previously shown to stimulate a protective response to influenza A infection using M2e as a mixture of monomers, dimers and multimers (L-M2e1-HD/MPL). Our results using these modified forms of M2e produced 90%-100% survival following lethal challenge with H1N1 (A/PR/8/34) in both inbred BALB/c and outbred Swiss Webster mice vaccinated with a truncated monomeric form of the M2 protein, M2e[SUB]1-15[/SUB] in liposomes. These observations show that a tetrameric configuration is not required to elicit significant protection when the M2e antigen is formulated in immunogenic liposomes and further, that the first 15 amino acids of M2e likely play a primary role in providing the protective immune response.
[h=4]KEYWORDS:[/h] influenza M2e; liposomes; vaccination
PMID: 28922045 DOI: 10.1080/08982104.2017.1381708