tetano
Editor, Senior Moderator
Life Sci. 2018 May 31. pii: S0024-3205(18)30336-9. doi: 10.1016/j.lfs.2018.05.056. [Epub ahead of print]
[h=1]Mouse strain and sex as determinants of immune response to trivalent influenza vaccine.[/h] Petrović R[SUP]1[/SUP], Bufan B[SUP]2[/SUP], Arsenović-Ranin N[SUP]2[/SUP], ?ivković I[SUP]1[/SUP], Minić R[SUP]1[/SUP], Radojević K[SUP]1[/SUP], Leposavić G[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]AIMS:[/h] The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV).
[h=4]MAIN METHODS:[/h] C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-γ and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively.
[h=4]KEY FINDINGS:[/h] BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sex-matched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-γ than those from C57BL/6 mice.
[h=4]SIGNIFICANCE:[/h] The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.
Copyright ? 2017. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Antibody response; Germinal center reaction; Influenza vaccine; Mouse genetic background; Sex differences
PMID: 29859986 DOI: 10.1016/j.lfs.2018.05.056
[h=1]Mouse strain and sex as determinants of immune response to trivalent influenza vaccine.[/h] Petrović R[SUP]1[/SUP], Bufan B[SUP]2[/SUP], Arsenović-Ranin N[SUP]2[/SUP], ?ivković I[SUP]1[/SUP], Minić R[SUP]1[/SUP], Radojević K[SUP]1[/SUP], Leposavić G[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]AIMS:[/h] The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV).
[h=4]MAIN METHODS:[/h] C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-γ and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively.
[h=4]KEY FINDINGS:[/h] BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sex-matched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-γ than those from C57BL/6 mice.
[h=4]SIGNIFICANCE:[/h] The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.
Copyright ? 2017. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Antibody response; Germinal center reaction; Influenza vaccine; Mouse genetic background; Sex differences
PMID: 29859986 DOI: 10.1016/j.lfs.2018.05.056