tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2011 Nov 30. [Epub ahead of print]
Mucosal and Systemic Immune Responses Induced by Recombinant Lactobacillus Expressing the Hemagglutinin of the Avian Influenza Virus.
Wang Z, Yu Q, Gao J, Yang Q.
Source
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, PR China.
Abstract
To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed two recombinant lactobacilli (LA4356-pH and DLD17-pH) that express the foreign HPAI protein, hemagglutinin 1 (HA(1)). The mucosal and systemic immune responses that are triggered by these two recombinant lactobacilli following the oral administration to BALB/c mice were evaluated. The results showed that both LA4356-pH and DLD17-pH could significantly increase the specific anti-HA(1) IgA antibody level in the mucosa and the anti-HA(1) IgG level in sera as well as stimulate the splenic lymphocyte proliferative reaction through the increased expression of IL-4. Compared with LA4356-pH, DLD17-pH was more effective in inducing the systemic and mucosal immune responses with higher anti-HA1-specific IgA and IgG levels. Therefore, DLD17-pH could be a promising oral vaccine candidate against HPAI.
PMID:
22131355
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22131355
Mucosal and Systemic Immune Responses Induced by Recombinant Lactobacillus Expressing the Hemagglutinin of the Avian Influenza Virus.
Wang Z, Yu Q, Gao J, Yang Q.
Source
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, PR China.
Abstract
To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed two recombinant lactobacilli (LA4356-pH and DLD17-pH) that express the foreign HPAI protein, hemagglutinin 1 (HA(1)). The mucosal and systemic immune responses that are triggered by these two recombinant lactobacilli following the oral administration to BALB/c mice were evaluated. The results showed that both LA4356-pH and DLD17-pH could significantly increase the specific anti-HA(1) IgA antibody level in the mucosa and the anti-HA(1) IgG level in sera as well as stimulate the splenic lymphocyte proliferative reaction through the increased expression of IL-4. Compared with LA4356-pH, DLD17-pH was more effective in inducing the systemic and mucosal immune responses with higher anti-HA1-specific IgA and IgG levels. Therefore, DLD17-pH could be a promising oral vaccine candidate against HPAI.
PMID:
22131355
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22131355