tetano
Editor, Senior Moderator
Mucosal Immunol
. 2022 Jul 15.
doi: 10.1038/s41385-022-00545-4. Online ahead of print.
Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
Helen Stölting[SUP] 1 [/SUP], Laury Baillon[SUP] 2 [/SUP], Rebecca Frise[SUP] 2 [/SUP], Katie Bonner[SUP] 1 3 4 [/SUP], Richard J Hewitt[SUP] 1 4 [/SUP], Philip L Molyneaux[SUP] 1 4 [/SUP], Mindy L Gore[SUP] 1 [/SUP], Breathing Together Consortium; Wendy S Barclay[SUP] 2 [/SUP], Sejal Saglani[SUP] #[/SUP][SUP] 5 [/SUP], Clare M Lloyd[SUP] #[/SUP][SUP] 6 [/SUP]
Collaborators, Affiliations
Abstract
Children are less likely than adults to suffer severe symptoms when infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), while influenza A H1N1 severity is comparable across ages except for the very young or elderly. Airway epithelial cells play a vital role in the early defence against viruses via their barrier and immune functions. We investigated viral replication and immune responses in SARS-CoV-2-infected bronchial epithelial cells from healthy paediatric (n = 6; 2.5-5.6 years old) and adult (n = 4; 47-63 years old) subjects and compared cellular responses following infection with SARS-CoV-2 or Influenza A H1N1. While infection with either virus triggered robust transcriptional interferon responses, including induction of type I (IFNB1) and type III (IFNL1) interferons, markedly lower levels of interferons and inflammatory proteins (IL-6, IL-8) were released following SARS-CoV-2 compared to H1N1 infection. Only H1N1 infection caused disruption of the epithelial layer. Interestingly, H1N1 infection resulted in sustained upregulation of SARS-CoV-2 entry factors FURIN and NRP1. We did not find any differences in the epithelial response to SARS-CoV-2 infection between paediatric and adult cells. Overall, SARS-CoV-2 had diminished potential to replicate, affect morphology and evoke immune responses in bronchial epithelial cells compared to H1N1.
. 2022 Jul 15.
doi: 10.1038/s41385-022-00545-4. Online ahead of print.
Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
Helen Stölting[SUP] 1 [/SUP], Laury Baillon[SUP] 2 [/SUP], Rebecca Frise[SUP] 2 [/SUP], Katie Bonner[SUP] 1 3 4 [/SUP], Richard J Hewitt[SUP] 1 4 [/SUP], Philip L Molyneaux[SUP] 1 4 [/SUP], Mindy L Gore[SUP] 1 [/SUP], Breathing Together Consortium; Wendy S Barclay[SUP] 2 [/SUP], Sejal Saglani[SUP] #[/SUP][SUP] 5 [/SUP], Clare M Lloyd[SUP] #[/SUP][SUP] 6 [/SUP]
Collaborators, Affiliations
- PMID: 35840680
- DOI: 10.1038/s41385-022-00545-4
Abstract
Children are less likely than adults to suffer severe symptoms when infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), while influenza A H1N1 severity is comparable across ages except for the very young or elderly. Airway epithelial cells play a vital role in the early defence against viruses via their barrier and immune functions. We investigated viral replication and immune responses in SARS-CoV-2-infected bronchial epithelial cells from healthy paediatric (n = 6; 2.5-5.6 years old) and adult (n = 4; 47-63 years old) subjects and compared cellular responses following infection with SARS-CoV-2 or Influenza A H1N1. While infection with either virus triggered robust transcriptional interferon responses, including induction of type I (IFNB1) and type III (IFNL1) interferons, markedly lower levels of interferons and inflammatory proteins (IL-6, IL-8) were released following SARS-CoV-2 compared to H1N1 infection. Only H1N1 infection caused disruption of the epithelial layer. Interestingly, H1N1 infection resulted in sustained upregulation of SARS-CoV-2 entry factors FURIN and NRP1. We did not find any differences in the epithelial response to SARS-CoV-2 infection between paediatric and adult cells. Overall, SARS-CoV-2 had diminished potential to replicate, affect morphology and evoke immune responses in bronchial epithelial cells compared to H1N1.