tetano
Editor, Senior Moderator
J Immunol. 2014 Jun 23. pii: 1400222. [Epub ahead of print]
Mucosal Polyinosinic-Polycytidylic Acid Improves Protection Elicited by Replicating Influenza Vaccines via Enhanced Dendritic Cell Function and T Cell Immunity.
P?rez-Gir?n JV1, Belicha-Villanueva A2, Hassan E1, G?mez-Medina S1, Cruz JL1, L?dtke A1, Ruibal P1, Albrecht RA3, Garc?a-Sastre A4, Mu?oz-Fontela C5.
Author information
Abstract
Live-attenuated influenza vaccines (LAIVs) have the potential to generate CD8 T cell immunity that may limit the virulence of an antigenically shifted influenza strain in a population lacking protective Abs. However, current LAIVs exert limited T cell immunity restricted to the vaccine strains. One approach to improve LAIV-induced T cell responses is the use of specific adjuvants to enhance T cell priming by respiratory dendritic cells, but this hypothesis has not been addressed. In this study, we assessed the effect of the TLR3 ligand polyinosinic-polycytidylic acid (poly IC) on CD8 T cell immunity and protection elicited by LAIVs. Mucosal treatment with poly IC shortly after vaccination enhanced respiratory dendritic cell function, CD8 T cell formation, and production of neutralizing Abs. This adjuvant effect of poly IC was dependent on amplification of TLR3 signaling by nonhematopoietic radioresistant cells and enhanced mouse protection to homosubtypic, as well as heterosubtypic, virus challenge. Our findings indicate that mucosal TLR3 ligation may be used to improve CD8 T cell responses to replicating vaccines, which has implications for protection in the absence of pre-existing Ab immunity.
Copyright ? 2014 by The American Association of Immunologists, Inc.
PMID:
24958904
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24958904
Mucosal Polyinosinic-Polycytidylic Acid Improves Protection Elicited by Replicating Influenza Vaccines via Enhanced Dendritic Cell Function and T Cell Immunity.
P?rez-Gir?n JV1, Belicha-Villanueva A2, Hassan E1, G?mez-Medina S1, Cruz JL1, L?dtke A1, Ruibal P1, Albrecht RA3, Garc?a-Sastre A4, Mu?oz-Fontela C5.
Author information
Abstract
Live-attenuated influenza vaccines (LAIVs) have the potential to generate CD8 T cell immunity that may limit the virulence of an antigenically shifted influenza strain in a population lacking protective Abs. However, current LAIVs exert limited T cell immunity restricted to the vaccine strains. One approach to improve LAIV-induced T cell responses is the use of specific adjuvants to enhance T cell priming by respiratory dendritic cells, but this hypothesis has not been addressed. In this study, we assessed the effect of the TLR3 ligand polyinosinic-polycytidylic acid (poly IC) on CD8 T cell immunity and protection elicited by LAIVs. Mucosal treatment with poly IC shortly after vaccination enhanced respiratory dendritic cell function, CD8 T cell formation, and production of neutralizing Abs. This adjuvant effect of poly IC was dependent on amplification of TLR3 signaling by nonhematopoietic radioresistant cells and enhanced mouse protection to homosubtypic, as well as heterosubtypic, virus challenge. Our findings indicate that mucosal TLR3 ligation may be used to improve CD8 T cell responses to replicating vaccines, which has implications for protection in the absence of pre-existing Ab immunity.
Copyright ? 2014 by The American Association of Immunologists, Inc.
PMID:
24958904
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24958904