tetano
Editor, Senior Moderator
J Clin Microbiol. 2017 Oct 4. pii: JCM.00957-17. doi: 10.1128/JCM.00957-17. [Epub ahead of print]
[h=1]Multiplex RT-PCR for Simultaneous Surveillance of Influenza A and B Viruses.[/h] Zhou B[SUP]1,[/SUP][SUP]2[/SUP], Deng YM[SUP]3[/SUP], Barnes JR[SUP]4[/SUP], Sessions O[SUP]5[/SUP], Chou TW[SUP]1[/SUP], Wilson M[SUP]4[/SUP], Stark TJ[SUP]4[/SUP], Volk M[SUP]1[/SUP], Spirason N[SUP]6[/SUP], Halpin RA[SUP]2[/SUP], Kamaraj US[SUP]5[/SUP], Ding T[SUP]1[/SUP], Stockwell TB[SUP]2[/SUP], Salvatore M[SUP]7[/SUP], Ghedin E[SUP]1,[/SUP][SUP]7[/SUP], Barr IG[SUP]6[/SUP], Wentworth DE[SUP]8,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe; influenza A viruses intermittently cause pandemics. Sequence information from influenza genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction FluA/B Multiplex RT-PCR method that amplifies the most critical genomic segments (HA, NA, and M) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral types, subtypes, or lineages. Herein we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus positive specimens using multiple next-generation sequencing platforms.
Copyright ? 2017 American Society for Microbiology.
PMID: 28978683 DOI: 10.1128/JCM.00957-17
[h=1]Multiplex RT-PCR for Simultaneous Surveillance of Influenza A and B Viruses.[/h] Zhou B[SUP]1,[/SUP][SUP]2[/SUP], Deng YM[SUP]3[/SUP], Barnes JR[SUP]4[/SUP], Sessions O[SUP]5[/SUP], Chou TW[SUP]1[/SUP], Wilson M[SUP]4[/SUP], Stark TJ[SUP]4[/SUP], Volk M[SUP]1[/SUP], Spirason N[SUP]6[/SUP], Halpin RA[SUP]2[/SUP], Kamaraj US[SUP]5[/SUP], Ding T[SUP]1[/SUP], Stockwell TB[SUP]2[/SUP], Salvatore M[SUP]7[/SUP], Ghedin E[SUP]1,[/SUP][SUP]7[/SUP], Barr IG[SUP]6[/SUP], Wentworth DE[SUP]8,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe; influenza A viruses intermittently cause pandemics. Sequence information from influenza genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction FluA/B Multiplex RT-PCR method that amplifies the most critical genomic segments (HA, NA, and M) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral types, subtypes, or lineages. Herein we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus positive specimens using multiple next-generation sequencing platforms.
Copyright ? 2017 American Society for Microbiology.
PMID: 28978683 DOI: 10.1128/JCM.00957-17