tetano
Editor, Senior Moderator
Biosens Bioelectron. 2015 Oct 8;77:464-470. doi: 10.1016/j.bios.2015.10.002. [Epub ahead of print]
[h=1]Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay.[/h] Wu F[SUP]1[/SUP], Yuan H[SUP]2[/SUP], Zhou C[SUP]3[/SUP], Mao M[SUP]3[/SUP], Liu Q[SUP]2[/SUP], Shen H[SUP]3[/SUP], Cen Y[SUP]2[/SUP], Qin Z[SUP]4[/SUP], Ma L[SUP]5[/SUP], Song Li L[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Immunoassay; Influenza A virus; Quantum dots
PMID: 26454828 [PubMed - as supplied by publisher]
[h=1]Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay.[/h] Wu F[SUP]1[/SUP], Yuan H[SUP]2[/SUP], Zhou C[SUP]3[/SUP], Mao M[SUP]3[/SUP], Liu Q[SUP]2[/SUP], Shen H[SUP]3[/SUP], Cen Y[SUP]2[/SUP], Qin Z[SUP]4[/SUP], Ma L[SUP]5[/SUP], Song Li L[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Immunoassay; Influenza A virus; Quantum dots
PMID: 26454828 [PubMed - as supplied by publisher]