tetano
Editor, Senior Moderator
Vet Immunol Immunopathol. 2014 Dec 23. pii: S0165-2427(14)00311-0. doi: 10.1016/j.vetimm.2014.12.009. [Epub ahead of print]
[h=1]Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections.[/h] Cheng Y[SUP]1[/SUP], Huang Q[SUP]1[/SUP], Ji W[SUP]1[/SUP], Du B[SUP]1[/SUP], Fu Q[SUP]1[/SUP], An H[SUP]1[/SUP], Li J[SUP]2[/SUP], Wang H[SUP]1[/SUP], Yan Y[SUP]1[/SUP], Ding C[SUP]3[/SUP], Sun J[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Retinoic acid inducible gene I (RIG-I) is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs). Muscovy duck (Cairina moschata) is a large duck different from other species of ducks, and is more susceptible to some microbial pathogens. In this study, the Muscovy duck RIG-I gene (MdRIG-I) was identified. Quantitative RT-PCR showed that MdRIG-I mRNA was widely expressed in different tissues, especially in those with mucosa. RIG-I null DF-1 cells transfected with DNA constructs encoding MdRIG-I or CARDs domain can activate IRF-3 and NF-κB to up-regulated activity of IFN-β promoter. The components of the signaling pathway downstream of RIG-I in mammalian cells including IRF-3, NF-κB, IFN-β and the IFN-stimulated genes Mx-1, PKR and MDA5 were significantly up-regulated in CARDs-overexpressing-DF-1 cells. Implicating RIG-I in the antiviral response to an infection in vivo, we found that RIG-I expression in brain, spleen, lung and bursa were up-regulated in ducks challenged with H9N2 avian influenza virus (AIV), whose six internal genes were closely related to the H7N9 and H10N8 AIV. In vitro, DF-1 cells transfected with MdRIG-I plasmid can respond significantly to H9N2 AIV, evident through enhancement of IFN-β promoter activity and decreased virus titer. Altogether, these results indicated that MdRIG-I is a novel member of RLR gene family, engaging in the early stage of antiviral innate immunity.
Copyright ? 2014 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] DF-1; H9N2; IFN-β; Innate immune response; Muscovy duck; RIG-I
PMID: 25604129 [PubMed - as supplied by publisher]
[h=1]Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections.[/h] Cheng Y[SUP]1[/SUP], Huang Q[SUP]1[/SUP], Ji W[SUP]1[/SUP], Du B[SUP]1[/SUP], Fu Q[SUP]1[/SUP], An H[SUP]1[/SUP], Li J[SUP]2[/SUP], Wang H[SUP]1[/SUP], Yan Y[SUP]1[/SUP], Ding C[SUP]3[/SUP], Sun J[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Retinoic acid inducible gene I (RIG-I) is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs). Muscovy duck (Cairina moschata) is a large duck different from other species of ducks, and is more susceptible to some microbial pathogens. In this study, the Muscovy duck RIG-I gene (MdRIG-I) was identified. Quantitative RT-PCR showed that MdRIG-I mRNA was widely expressed in different tissues, especially in those with mucosa. RIG-I null DF-1 cells transfected with DNA constructs encoding MdRIG-I or CARDs domain can activate IRF-3 and NF-κB to up-regulated activity of IFN-β promoter. The components of the signaling pathway downstream of RIG-I in mammalian cells including IRF-3, NF-κB, IFN-β and the IFN-stimulated genes Mx-1, PKR and MDA5 were significantly up-regulated in CARDs-overexpressing-DF-1 cells. Implicating RIG-I in the antiviral response to an infection in vivo, we found that RIG-I expression in brain, spleen, lung and bursa were up-regulated in ducks challenged with H9N2 avian influenza virus (AIV), whose six internal genes were closely related to the H7N9 and H10N8 AIV. In vitro, DF-1 cells transfected with MdRIG-I plasmid can respond significantly to H9N2 AIV, evident through enhancement of IFN-β promoter activity and decreased virus titer. Altogether, these results indicated that MdRIG-I is a novel member of RLR gene family, engaging in the early stage of antiviral innate immunity.
Copyright ? 2014 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] DF-1; H9N2; IFN-β; Innate immune response; Muscovy duck; RIG-I
PMID: 25604129 [PubMed - as supplied by publisher]