tetano
Editor, Senior Moderator
Int Immunopharmacol. 2014 Jun 23. pii: S1567-5769(14)00227-6. doi: 10.1016/j.intimp.2014.06.013. [Epub ahead of print]
N-acetyl-l-cystine (NAC) protects against H9N2 swine influenza virus-induced acute lung injury.
Zhang RH1, Li CH1, Wang CL1, Xu MJ1, Tong X2, Dong W1, Liu BJ1, Wang GH1, Tian SF1.
Author information
Abstract
The antioxidant N-acetyl-l-cysteine (NAC) had been shown to inhibit replication of seasonal human influenza A viruses. Here, the effects of NAC on H9N2 swine influenza virus-induced acute lung injury (ALI) were investigated in mice. BALB/c mice were inoculated intranasally with 107 50% tissue culture infective doses (TCID50) of A/swine/HeBei/012/2008/(H9N2) viruses with or without NAC treatments to induce ALI model. The result showed that pulmonary inflammation, pulmonary edema, MPO activity, total cells, neutrophils, macrophages, TNF-α, IL-6, IL-1β and CXCL-10 in BALF were attenuated by NAC. Moreover, our data showed that NAC significantly inhibited the levels of TLR4 protein and TLR4 mRNA in the lungs. Pharmacological inhibitors of TLR4 (E5564) exerted similar effects like those determined for NAC in H9N2 swine influenza virus-infected mice. These results suggest that antioxidants like NAC represent a potential additional treatment option that could be considered in the case of an influenza A virus pandemic.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Acute lung injury; H9N2 swine influenza virus; N-acetyl-l-cystine (NAC); Toll-like receptor 4
PMID:
24968347
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24968347
N-acetyl-l-cystine (NAC) protects against H9N2 swine influenza virus-induced acute lung injury.
Zhang RH1, Li CH1, Wang CL1, Xu MJ1, Tong X2, Dong W1, Liu BJ1, Wang GH1, Tian SF1.
Author information
Abstract
The antioxidant N-acetyl-l-cysteine (NAC) had been shown to inhibit replication of seasonal human influenza A viruses. Here, the effects of NAC on H9N2 swine influenza virus-induced acute lung injury (ALI) were investigated in mice. BALB/c mice were inoculated intranasally with 107 50% tissue culture infective doses (TCID50) of A/swine/HeBei/012/2008/(H9N2) viruses with or without NAC treatments to induce ALI model. The result showed that pulmonary inflammation, pulmonary edema, MPO activity, total cells, neutrophils, macrophages, TNF-α, IL-6, IL-1β and CXCL-10 in BALF were attenuated by NAC. Moreover, our data showed that NAC significantly inhibited the levels of TLR4 protein and TLR4 mRNA in the lungs. Pharmacological inhibitors of TLR4 (E5564) exerted similar effects like those determined for NAC in H9N2 swine influenza virus-infected mice. These results suggest that antioxidants like NAC represent a potential additional treatment option that could be considered in the case of an influenza A virus pandemic.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Acute lung injury; H9N2 swine influenza virus; N-acetyl-l-cystine (NAC); Toll-like receptor 4
PMID:
24968347
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24968347