tetano
Editor, Senior Moderator
Nanomicro Lett
. 2021 Apr 13;13(1):109.
doi: 10.1007/s40820-021-00620-8.
Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection
Yong Yang[SUP] 1 2 3 [/SUP], Yusi Peng[SUP] 4 5 [/SUP], Chenglong Lin[SUP] 4 5 [/SUP], Li Long[SUP] 6 [/SUP], Jingying Hu[SUP] 7 [/SUP], Jun He[SUP] 8 9 [/SUP], Hui Zeng[SUP] 10 [/SUP], Zhengren Huang[SUP] 11 [/SUP], Zhi-Yuan Li[SUP] 6 [/SUP], Masaki Tanemura[SUP] 12 [/SUP], Jianlin Shi[SUP] 4 [/SUP], John R Lombardi[SUP] 13 [/SUP], Xiaoying Luo[SUP] 14 [/SUP]
Affiliations
Abstract
The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10[SUP]6[/SUP]-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10[SUP]9[/SUP]-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL[SUP]-1[/SUP] for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.
Keywords: Human ACE2; SARS-CoV-2; SERS; Single-virus detection; “Virus-trap” nanostructure.
. 2021 Apr 13;13(1):109.
doi: 10.1007/s40820-021-00620-8.
Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection
Yong Yang[SUP] 1 2 3 [/SUP], Yusi Peng[SUP] 4 5 [/SUP], Chenglong Lin[SUP] 4 5 [/SUP], Li Long[SUP] 6 [/SUP], Jingying Hu[SUP] 7 [/SUP], Jun He[SUP] 8 9 [/SUP], Hui Zeng[SUP] 10 [/SUP], Zhengren Huang[SUP] 11 [/SUP], Zhi-Yuan Li[SUP] 6 [/SUP], Masaki Tanemura[SUP] 12 [/SUP], Jianlin Shi[SUP] 4 [/SUP], John R Lombardi[SUP] 13 [/SUP], Xiaoying Luo[SUP] 14 [/SUP]
Affiliations
- PMID: 34138316
- DOI: 10.1007/s40820-021-00620-8
Abstract
The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10[SUP]6[/SUP]-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10[SUP]9[/SUP]-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL[SUP]-1[/SUP] for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.
Keywords: Human ACE2; SARS-CoV-2; SERS; Single-virus detection; “Virus-trap” nanostructure.