tetano
Editor, Senior Moderator
Nat Biomed Eng
. 2022 Aug 8.
doi: 10.1038/s41551-022-00919-w. Online ahead of print.
A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma
Devora Najjar[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Joshua Rainbow[SUP] #[/SUP][SUP] 1 4 [/SUP], Sanjay Sharma Timilsina[SUP] #[/SUP][SUP] 1 [/SUP], Pawan Jolly[SUP] #[/SUP][SUP] 1 [/SUP], Helena de Puig[SUP] #[/SUP][SUP] 1 2 [/SUP], Mohamed Yafia[SUP] 1 [/SUP], Nolan Durr[SUP] 1 [/SUP], Hani Sallum[SUP] 1 [/SUP], Galit Alter[SUP] 5 [/SUP], Jonathan Z Li[SUP] 6 [/SUP], Xu G Yu[SUP] 5 6 [/SUP], David R Walt[SUP] 1 7 8 [/SUP], Joseph A Paradiso[SUP] 3 [/SUP], Pedro Estrela[SUP] 4 [/SUP], James J Collins[SUP] 9 10 11 [/SUP], Donald E Ingber[SUP] 12 13 14 15 [/SUP]
Affiliations
Abstract
Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity.
. 2022 Aug 8.
doi: 10.1038/s41551-022-00919-w. Online ahead of print.
A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma
Devora Najjar[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Joshua Rainbow[SUP] #[/SUP][SUP] 1 4 [/SUP], Sanjay Sharma Timilsina[SUP] #[/SUP][SUP] 1 [/SUP], Pawan Jolly[SUP] #[/SUP][SUP] 1 [/SUP], Helena de Puig[SUP] #[/SUP][SUP] 1 2 [/SUP], Mohamed Yafia[SUP] 1 [/SUP], Nolan Durr[SUP] 1 [/SUP], Hani Sallum[SUP] 1 [/SUP], Galit Alter[SUP] 5 [/SUP], Jonathan Z Li[SUP] 6 [/SUP], Xu G Yu[SUP] 5 6 [/SUP], David R Walt[SUP] 1 7 8 [/SUP], Joseph A Paradiso[SUP] 3 [/SUP], Pedro Estrela[SUP] 4 [/SUP], James J Collins[SUP] 9 10 11 [/SUP], Donald E Ingber[SUP] 12 13 14 15 [/SUP]
Affiliations
- PMID: 35941191
- DOI: 10.1038/s41551-022-00919-w
Abstract
Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity.