tetano
Editor, Senior Moderator
Nat Biomed Eng
. 2024 May 7.
doi: 10.1038/s41551-024-01208-4. Online ahead of print. Dendritic-cell-targeting virus-like particles as potent mRNA vaccine carriers
Di Yin[SUP] #[/SUP][SUP] 1 [/SUP], Yiye Zhong[SUP] #[/SUP][SUP] 1 [/SUP], Sikai Ling[SUP] #[/SUP][SUP] 1 2 [/SUP], Sicong Lu[SUP] #[/SUP][SUP] 1 [/SUP], Xiaoyuan Wang[SUP] #[/SUP][SUP] 2 [/SUP], Zhuofan Jiang[SUP] 1 [/SUP], Jie Wang[SUP] 1 [/SUP], Yao Dai[SUP] 1 [/SUP], Xiaolong Tian[SUP] 3 [/SUP], Qijing Huang[SUP] 1 [/SUP], Xingbo Wang[SUP] 4 [/SUP], Junsong Chen[SUP] 1 [/SUP], Ziying Li[SUP] 1 [/SUP], Yang Li[SUP] 1 [/SUP], Zhijue Xu[SUP] 1 [/SUP], Hewei Jiang[SUP] 1 [/SUP], Yuqing Wu[SUP] 5 [/SUP], Yi Shi[SUP] 6 [/SUP], Quanjun Wang[SUP] 7 [/SUP], Jianjiang Xu[SUP] 5 [/SUP], Wei Hong[SUP] 8 9 [/SUP], Heng Xue[SUP] 8 9 [/SUP], Hang Yang[SUP] 8 9 10 [/SUP], Yan Zhang[SUP] 1 [/SUP], Lintai Da[SUP] 1 [/SUP], Ze-Guang Han[SUP] 1 [/SUP], Sheng-Ce Tao[SUP] 1 [/SUP], Ruijiao Dong[SUP] 1 [/SUP], Tianlei Ying[SUP] 3 [/SUP], Jiaxu Hong[SUP] 11 [/SUP], Yujia Cai[SUP] 12 [/SUP]
Affiliations
Messenger RNA vaccines lack specificity for dendritic cells (DCs)-the most effective cells at antigen presentation. Here we report the design and performance of a DC-targeting virus-like particle pseudotyped with an engineered Sindbis-virus glycoprotein that recognizes a surface protein on DCs, and packaging mRNA encoding for the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or for the glycoproteins B and D of herpes simplex virus 1. Injection of the DC-targeting SARS-CoV-2 mRNA vaccine in the footpad of mice led to substantially higher and durable antigen-specific immunoglobulin-G titres and cellular immune responses than untargeted virus-like particles and lipid-nanoparticle formulations. The vaccines also protected the mice from infection with SARS-CoV-2 or with herpes simplex virus 1. Virus-like particles with preferential uptake by DCs may facilitate the development of potent prophylactic and therapeutic vaccines.
. 2024 May 7.
doi: 10.1038/s41551-024-01208-4. Online ahead of print. Dendritic-cell-targeting virus-like particles as potent mRNA vaccine carriers
Di Yin[SUP] #[/SUP][SUP] 1 [/SUP], Yiye Zhong[SUP] #[/SUP][SUP] 1 [/SUP], Sikai Ling[SUP] #[/SUP][SUP] 1 2 [/SUP], Sicong Lu[SUP] #[/SUP][SUP] 1 [/SUP], Xiaoyuan Wang[SUP] #[/SUP][SUP] 2 [/SUP], Zhuofan Jiang[SUP] 1 [/SUP], Jie Wang[SUP] 1 [/SUP], Yao Dai[SUP] 1 [/SUP], Xiaolong Tian[SUP] 3 [/SUP], Qijing Huang[SUP] 1 [/SUP], Xingbo Wang[SUP] 4 [/SUP], Junsong Chen[SUP] 1 [/SUP], Ziying Li[SUP] 1 [/SUP], Yang Li[SUP] 1 [/SUP], Zhijue Xu[SUP] 1 [/SUP], Hewei Jiang[SUP] 1 [/SUP], Yuqing Wu[SUP] 5 [/SUP], Yi Shi[SUP] 6 [/SUP], Quanjun Wang[SUP] 7 [/SUP], Jianjiang Xu[SUP] 5 [/SUP], Wei Hong[SUP] 8 9 [/SUP], Heng Xue[SUP] 8 9 [/SUP], Hang Yang[SUP] 8 9 10 [/SUP], Yan Zhang[SUP] 1 [/SUP], Lintai Da[SUP] 1 [/SUP], Ze-Guang Han[SUP] 1 [/SUP], Sheng-Ce Tao[SUP] 1 [/SUP], Ruijiao Dong[SUP] 1 [/SUP], Tianlei Ying[SUP] 3 [/SUP], Jiaxu Hong[SUP] 11 [/SUP], Yujia Cai[SUP] 12 [/SUP]
Affiliations
- PMID: 38714892
- DOI: 10.1038/s41551-024-01208-4
Messenger RNA vaccines lack specificity for dendritic cells (DCs)-the most effective cells at antigen presentation. Here we report the design and performance of a DC-targeting virus-like particle pseudotyped with an engineered Sindbis-virus glycoprotein that recognizes a surface protein on DCs, and packaging mRNA encoding for the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or for the glycoproteins B and D of herpes simplex virus 1. Injection of the DC-targeting SARS-CoV-2 mRNA vaccine in the footpad of mice led to substantially higher and durable antigen-specific immunoglobulin-G titres and cellular immune responses than untargeted virus-like particles and lipid-nanoparticle formulations. The vaccines also protected the mice from infection with SARS-CoV-2 or with herpes simplex virus 1. Virus-like particles with preferential uptake by DCs may facilitate the development of potent prophylactic and therapeutic vaccines.