tetano
Editor, Senior Moderator
Nat Biotechnol
. 2022 Apr 28.
doi: 10.1038/s41587-022-01280-8. Online ahead of print.
Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
Jason Z Zhang[SUP] #[/SUP][SUP] 1 2 [/SUP], Hsien-Wei Yeh[SUP] #[/SUP][SUP] 3 4 [/SUP], Alexandra C Walls[SUP] 3 [/SUP], Basile I M Wicky[SUP] 3 4 [/SUP], Kaitlin R Sprouse[SUP] 3 [/SUP], Laura A VanBlargan[SUP] 5 [/SUP], Rebecca Treger[SUP] 6 [/SUP], Alfredo Quijano-Rubio[SUP] 4 7 [/SUP], Minh N Pham[SUP] 3 4 [/SUP], John C Kraft[SUP] 3 4 [/SUP], Ian C Haydon[SUP] 4 [/SUP], Wei Yang[SUP] 3 4 [/SUP], Michelle DeWitt[SUP] 4 [/SUP], John E Bowen[SUP] 3 [/SUP], Cameron M Chow[SUP] 4 [/SUP], Lauren Carter[SUP] 4 [/SUP], Rashmi Ravichandran[SUP] 4 [/SUP], Mark H Wener[SUP] 6 [/SUP], Lance Stewart[SUP] 4 [/SUP], David Veesler[SUP] 3 8 [/SUP], Michael S Diamond[SUP] 5 9 10 11 [/SUP], Alexander L Greninger[SUP] 6 12 [/SUP], David M Koelle[SUP] 6 12 13 14 15 [/SUP], David Baker[SUP] 16 17 18 [/SUP]
Affiliations
Abstract
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.
. 2022 Apr 28.
doi: 10.1038/s41587-022-01280-8. Online ahead of print.
Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
Jason Z Zhang[SUP] #[/SUP][SUP] 1 2 [/SUP], Hsien-Wei Yeh[SUP] #[/SUP][SUP] 3 4 [/SUP], Alexandra C Walls[SUP] 3 [/SUP], Basile I M Wicky[SUP] 3 4 [/SUP], Kaitlin R Sprouse[SUP] 3 [/SUP], Laura A VanBlargan[SUP] 5 [/SUP], Rebecca Treger[SUP] 6 [/SUP], Alfredo Quijano-Rubio[SUP] 4 7 [/SUP], Minh N Pham[SUP] 3 4 [/SUP], John C Kraft[SUP] 3 4 [/SUP], Ian C Haydon[SUP] 4 [/SUP], Wei Yang[SUP] 3 4 [/SUP], Michelle DeWitt[SUP] 4 [/SUP], John E Bowen[SUP] 3 [/SUP], Cameron M Chow[SUP] 4 [/SUP], Lauren Carter[SUP] 4 [/SUP], Rashmi Ravichandran[SUP] 4 [/SUP], Mark H Wener[SUP] 6 [/SUP], Lance Stewart[SUP] 4 [/SUP], David Veesler[SUP] 3 8 [/SUP], Michael S Diamond[SUP] 5 9 10 11 [/SUP], Alexander L Greninger[SUP] 6 12 [/SUP], David M Koelle[SUP] 6 12 13 14 15 [/SUP], David Baker[SUP] 16 17 18 [/SUP]
Affiliations
- PMID: 35484405
- DOI: 10.1038/s41587-022-01280-8
Abstract
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.