tetano
Editor, Senior Moderator
Nat Commun
. 2024 Feb 2;15(1):997.
doi: 10.1038/s41467-024-45180-8. A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection
Lorena M Coria[SUP] 1 2 [/SUP], Juan Manuel Rodriguez[SUP] 3 4 [/SUP], Agostina Demaria[SUP] 5 6 [/SUP], Laura A Bruno[SUP] 5 6 [/SUP], Mayra Rios Medrano[SUP] 5 6 [/SUP], Celeste Pueblas Castro[SUP] 5 6 [/SUP], Eliana F Castro[SUP] 5 6 [/SUP], Sabrina A Del Priore[SUP] 3 [/SUP], Andres C Hernando Insua[SUP] 3 4 [/SUP], Ingrid G Kaufmann[SUP] 3 [/SUP], Lucas M Saposnik[SUP] 5 6 [/SUP], William B Stone[SUP] 7 [/SUP], Lineia Prado[SUP] 5 6 [/SUP], Ulises S Notaro[SUP] 8 [/SUP], Ayelen N Amweg[SUP] 8 [/SUP], Pablo U Diaz[SUP] 8 [/SUP], Martin Avaro[SUP] 9 [/SUP], Hugo Ortega[SUP] 8 [/SUP], Ana Ceballos[SUP] 10 [/SUP], Valeria Krum[SUP] 3 [/SUP], Francisco M Zurvarra[SUP] 3 4 [/SUP], Johanna E Sidabra[SUP] 3 [/SUP], Ignacio Drehe[SUP] 3 [/SUP], Jonathan A Baqué[SUP] 3 [/SUP], Mariana Li Causi[SUP] 3 [/SUP], Analia V De Nichilo[SUP] 3 4 [/SUP], Cristian J Payes[SUP] 3 [/SUP], Teresa Southard[SUP] 7 [/SUP], Julio C Vega[SUP] 11 [/SUP], Albert J Auguste[SUP] 7 12 [/SUP], Diego E Álvarez[SUP] 5 6 [/SUP], Juan M Flo[SUP] 3 [/SUP], Karina A Pasquevich[SUP] 5 6 [/SUP], Juliana Cassataro[SUP] 13 14 [/SUP]
Affiliations
In the context of continuous emergence of SARS-CoV-2 variants of concern (VOCs), one strategy to prevent the severe outcomes of COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies of a RBD vaccine derived from the Gamma SARS-CoV-2 variant adjuvanted with Alum. The Gamma-adapted RBD vaccine is more immunogenic than the Ancestral RBD vaccine in terms of inducing broader neutralizing antibodies. The Gamma RBD presents more immunogenic B-cell restricted epitopes and induces a higher proportion of specific-B cells and plasmablasts than the Ancestral RBD version. The Gamma-adapted vaccine induces antigen specific T cell immune responses and confers protection against Ancestral and Omicron BA.5 SARS-CoV-2 challenge in mice. Moreover, the Gamma RBD vaccine induces higher and broader neutralizing antibody activity than homologous booster vaccination in mice previously primed with different SARS-CoV-2 vaccine platforms. Our study indicates that the adjuvanted Gamma RBD vaccine is highly immunogenic and a broad-spectrum vaccine candidate.
. 2024 Feb 2;15(1):997.
doi: 10.1038/s41467-024-45180-8. A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection
Lorena M Coria[SUP] 1 2 [/SUP], Juan Manuel Rodriguez[SUP] 3 4 [/SUP], Agostina Demaria[SUP] 5 6 [/SUP], Laura A Bruno[SUP] 5 6 [/SUP], Mayra Rios Medrano[SUP] 5 6 [/SUP], Celeste Pueblas Castro[SUP] 5 6 [/SUP], Eliana F Castro[SUP] 5 6 [/SUP], Sabrina A Del Priore[SUP] 3 [/SUP], Andres C Hernando Insua[SUP] 3 4 [/SUP], Ingrid G Kaufmann[SUP] 3 [/SUP], Lucas M Saposnik[SUP] 5 6 [/SUP], William B Stone[SUP] 7 [/SUP], Lineia Prado[SUP] 5 6 [/SUP], Ulises S Notaro[SUP] 8 [/SUP], Ayelen N Amweg[SUP] 8 [/SUP], Pablo U Diaz[SUP] 8 [/SUP], Martin Avaro[SUP] 9 [/SUP], Hugo Ortega[SUP] 8 [/SUP], Ana Ceballos[SUP] 10 [/SUP], Valeria Krum[SUP] 3 [/SUP], Francisco M Zurvarra[SUP] 3 4 [/SUP], Johanna E Sidabra[SUP] 3 [/SUP], Ignacio Drehe[SUP] 3 [/SUP], Jonathan A Baqué[SUP] 3 [/SUP], Mariana Li Causi[SUP] 3 [/SUP], Analia V De Nichilo[SUP] 3 4 [/SUP], Cristian J Payes[SUP] 3 [/SUP], Teresa Southard[SUP] 7 [/SUP], Julio C Vega[SUP] 11 [/SUP], Albert J Auguste[SUP] 7 12 [/SUP], Diego E Álvarez[SUP] 5 6 [/SUP], Juan M Flo[SUP] 3 [/SUP], Karina A Pasquevich[SUP] 5 6 [/SUP], Juliana Cassataro[SUP] 13 14 [/SUP]
Affiliations
- PMID: 38307851
- PMCID: PMC10837449
- DOI: 10.1038/s41467-024-45180-8
In the context of continuous emergence of SARS-CoV-2 variants of concern (VOCs), one strategy to prevent the severe outcomes of COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies of a RBD vaccine derived from the Gamma SARS-CoV-2 variant adjuvanted with Alum. The Gamma-adapted RBD vaccine is more immunogenic than the Ancestral RBD vaccine in terms of inducing broader neutralizing antibodies. The Gamma RBD presents more immunogenic B-cell restricted epitopes and induces a higher proportion of specific-B cells and plasmablasts than the Ancestral RBD version. The Gamma-adapted vaccine induces antigen specific T cell immune responses and confers protection against Ancestral and Omicron BA.5 SARS-CoV-2 challenge in mice. Moreover, the Gamma RBD vaccine induces higher and broader neutralizing antibody activity than homologous booster vaccination in mice previously primed with different SARS-CoV-2 vaccine platforms. Our study indicates that the adjuvanted Gamma RBD vaccine is highly immunogenic and a broad-spectrum vaccine candidate.