tetano
Editor, Senior Moderator
Nat Commun
. 2025 Aug 2;16(1):7103.
doi: 10.1038/s41467-025-62040-1. Broad neuraminidase antibodies confer protection against seasonal and avian influenza viruses
Kexin Lv[SUP] #[/SUP][SUP] 1 [/SUP], Xiaoman Li[SUP] #[/SUP][SUP] 2 [/SUP], Xingchen Zhu[SUP] #[/SUP][SUP] 1 [/SUP], Jian-Piao Cai[SUP] #[/SUP][SUP] 3 [/SUP], Shuning Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yuzhu Sun[SUP] 1 [/SUP], Lin Liu[SUP] 1 [/SUP], Xiaoyu Cai[SUP] 1 [/SUP], Rui Cao[SUP] 1 [/SUP], Mengxin Xu[SUP] 1 [/SUP], Xinyu Yue[SUP] 1 [/SUP], Yanmei Zhai[SUP] 1 [/SUP], Wanyu Luo[SUP] 1 [/SUP], Hongjie Lu[SUP] 1 [/SUP], Ruiying Li[SUP] 1 [/SUP], Haoting Mai[SUP] 1 [/SUP], Lei Deng[SUP] 4 [/SUP], Feng Ye[SUP] 5 [/SUP], Shifeng Chen[SUP] 5 [/SUP], Mang Shi[SUP] 6 [/SUP], Huanle Luo[SUP] 1 [/SUP], Xinquan Wang[SUP] 2 [/SUP], Shuofeng Yuan[SUP] 7 [/SUP], Yuelong Shu[SUP] 8 9 [/SUP], Jiwan Ge[SUP] 10 [/SUP], Yao-Qing Chen[SUP] 11 12 13 [/SUP]
Affiliations
Neuraminidase (NA) is a critical target for universal influenza vaccines and therapeutic antibodies, yet its antigenic landscape remains incompletely understood. Here we identify two broadly cross-protective monoclonal antibodies, CAV-F6 and CAV-F34, from influenza-infected individuals. These antibodies inhibit NA enzymatic activity across multiple subtypes and confer protection against seasonal influenza in female mouse models. Importantly, the two antibodies also neutralize emerging avian strains, including recent bovine H5N1 and H7N9 strains, both with pandemic potential. Structural studies reveal that both antibodies target conserved regions of the NA active site via HCDR3, blocking sialic acid interaction. Furthermore, we observe distinct occupancy for the two antibodies on N2 tetramer, which is likely due to differences in binding affinity. Our findings provide molecular insights into NA-targeted immunity and offer a foundation for developing broadly protective influenza vaccines and therapeutics.
. 2025 Aug 2;16(1):7103.
doi: 10.1038/s41467-025-62040-1. Broad neuraminidase antibodies confer protection against seasonal and avian influenza viruses
Kexin Lv[SUP] #[/SUP][SUP] 1 [/SUP], Xiaoman Li[SUP] #[/SUP][SUP] 2 [/SUP], Xingchen Zhu[SUP] #[/SUP][SUP] 1 [/SUP], Jian-Piao Cai[SUP] #[/SUP][SUP] 3 [/SUP], Shuning Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yuzhu Sun[SUP] 1 [/SUP], Lin Liu[SUP] 1 [/SUP], Xiaoyu Cai[SUP] 1 [/SUP], Rui Cao[SUP] 1 [/SUP], Mengxin Xu[SUP] 1 [/SUP], Xinyu Yue[SUP] 1 [/SUP], Yanmei Zhai[SUP] 1 [/SUP], Wanyu Luo[SUP] 1 [/SUP], Hongjie Lu[SUP] 1 [/SUP], Ruiying Li[SUP] 1 [/SUP], Haoting Mai[SUP] 1 [/SUP], Lei Deng[SUP] 4 [/SUP], Feng Ye[SUP] 5 [/SUP], Shifeng Chen[SUP] 5 [/SUP], Mang Shi[SUP] 6 [/SUP], Huanle Luo[SUP] 1 [/SUP], Xinquan Wang[SUP] 2 [/SUP], Shuofeng Yuan[SUP] 7 [/SUP], Yuelong Shu[SUP] 8 9 [/SUP], Jiwan Ge[SUP] 10 [/SUP], Yao-Qing Chen[SUP] 11 12 13 [/SUP]
Affiliations
- PMID: 40753167
- PMCID: PMC12318061
- DOI: 10.1038/s41467-025-62040-1
Neuraminidase (NA) is a critical target for universal influenza vaccines and therapeutic antibodies, yet its antigenic landscape remains incompletely understood. Here we identify two broadly cross-protective monoclonal antibodies, CAV-F6 and CAV-F34, from influenza-infected individuals. These antibodies inhibit NA enzymatic activity across multiple subtypes and confer protection against seasonal influenza in female mouse models. Importantly, the two antibodies also neutralize emerging avian strains, including recent bovine H5N1 and H7N9 strains, both with pandemic potential. Structural studies reveal that both antibodies target conserved regions of the NA active site via HCDR3, blocking sialic acid interaction. Furthermore, we observe distinct occupancy for the two antibodies on N2 tetramer, which is likely due to differences in binding affinity. Our findings provide molecular insights into NA-targeted immunity and offer a foundation for developing broadly protective influenza vaccines and therapeutics.