tetano
Editor, Senior Moderator
Nat Commun
. 2020 Dec 3;11(1):6201.
doi: 10.1038/s41467-020-19925-0.
Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts
Karina Helena Morais Cardozo[SUP] 1 [/SUP], Adriana Lebkuchen[SUP] 1 [/SUP], Guilherme Gon?alves Okai[SUP] 1 [/SUP], Rodrigo Andrade Schuch[SUP] 1 [/SUP], Luciana Godoy Viana[SUP] 1 [/SUP], Aline Nogueira Olive[SUP] 1 [/SUP], Carolina Dos Santos Lazari[SUP] 2 [/SUP], Ana Maria Fraga[SUP] 1 [/SUP], Celso Francisco Hernandes Granato[SUP] 2 [/SUP], Maria Carolina Tostes Pint?o[SUP] 1 [/SUP], Valdemir Melechco Carvalho[SUP] 3 [/SUP]
Affiliations
Abstract
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 96 samples within 4 hours. A TFC-MS system allows multiplexed analysis of 4 samples within 10 min, enabling the processing of more than 500 samples per day. We validate this method qualitatively (Tier 3) and quantitatively (Tier 1) using 985 specimens previously analyzed by real-time RT-PCR, and detect up to 84% of the positive cases with up to 97% specificity. The presented strategy has high sample stability and should be considered as an option for SARS-CoV-2 testing in large populations.
. 2020 Dec 3;11(1):6201.
doi: 10.1038/s41467-020-19925-0.
Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts
Karina Helena Morais Cardozo[SUP] 1 [/SUP], Adriana Lebkuchen[SUP] 1 [/SUP], Guilherme Gon?alves Okai[SUP] 1 [/SUP], Rodrigo Andrade Schuch[SUP] 1 [/SUP], Luciana Godoy Viana[SUP] 1 [/SUP], Aline Nogueira Olive[SUP] 1 [/SUP], Carolina Dos Santos Lazari[SUP] 2 [/SUP], Ana Maria Fraga[SUP] 1 [/SUP], Celso Francisco Hernandes Granato[SUP] 2 [/SUP], Maria Carolina Tostes Pint?o[SUP] 1 [/SUP], Valdemir Melechco Carvalho[SUP] 3 [/SUP]
Affiliations
- PMID: 33273458
- DOI: 10.1038/s41467-020-19925-0
Abstract
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. Here, we develop a high-throughput targeted proteomics assay to detect SARS-CoV-2 nucleoprotein peptides directly from nasopharyngeal and oropharyngeal swabs. A modified magnetic particle-based proteomics approach implemented on a robotic liquid handler enables fully automated preparation of 96 samples within 4 hours. A TFC-MS system allows multiplexed analysis of 4 samples within 10 min, enabling the processing of more than 500 samples per day. We validate this method qualitatively (Tier 3) and quantitatively (Tier 1) using 985 specimens previously analyzed by real-time RT-PCR, and detect up to 84% of the positive cases with up to 97% specificity. The presented strategy has high sample stability and should be considered as an option for SARS-CoV-2 testing in large populations.